e84 dental materials 29S (2013) e1–e96 171 Effect of cements ZOE on bond strength self adhesive resin cements M. Schroeder 1,∗ , E. Carvalho 2 , C.N. Carvalho 3 , W.L. Souza 2 , A.D. Loguercio 4 , J. Bauer 2 1 UFRJ, Rio De Janeiro, Brazil 2 UFMA, São Luis, MA, Brazil 3 USP, São Paulo, Brazil 4 Universidade Estadual De Ponta Grossa, Ponta Grossa, Paraná, Brazil Purpose: The aim of this study is to evaluate the influence of the use of provisional cementations containing Zinc Oxide and Eugenol on bond strength of self-adhesive resin cements to dentin. Methods and materials: Thirty molars were divided into 6 groups (n = 5). The materials used were: RelyX U100 (3MESPE) and Panavia F 2.0 (Kuraray) prior to permanent cementation, eugenol-containing provisional cements Provy (Dentsply) and eugenol-free RelyX Temp NE (3MESPE). To the temporary cementation discs acrylic resin were used in temporary cementation and kept for 7 days in distilled water at 37 ◦ C for 24 h. After this period, the crown and the temporary cement were removed and the teeth are cleaned. The permanent cementation of crowns composite resin (Herculite DA 3.5, Keer) was made with 6 mm in height and cemented with resin cements according manufacturer’s. The buccal, lingual and proximal are light polymerization with a halogen lamp unit for 40 s each side. The specimens were stored in distilled water at 37 ◦ C for 24 h and then sectioned into sticks approximately 0.8 mm. These were subjected to testing microtensile at the speed of 0.5 mm/min in a universal testing machine (Instron 3342). The data were statistically analyzed using two-way ANOVA and Tukey’s tests (=0.05). After the tests, an analysis of fractured specimens will be held in stereomicroscope (10×) and MEV. Results: The cement Panavia showed high mean microten- sile bond strength (32.81 ± 4.46) in control group. The presence of provisional cements reduces the bond strength only to Panavia cements. The U100 groups show the lowers bond strength values. Fractographic analysis shows the dentin–adhesive interface failure for all groups. Conclusion: The provisional cements must be avoided when used self-adhesive cements materials. http://dx.doi.org/10.1016/j.dental.2013.08.172 172 Microtensile bond strength of self-etching adhesives on caries affected dentin and normal dentin S. Shibata 1,∗ , L.N. Baratieri 1 , L.C.C. Vieria 1 , J. Fu 2 , S. Hoshika 2 , Y. Matsuda 2 , H. Sano 2 1 Federal University of Santa Catarina, Brazil 2 Hokkaido University, Japan Purpose: The aim of study was to compare the microten- sile bond strength (TBS) of 4 self-etch adhesives (Clearfil Mega Bond and 4MTB-200, Kuraray Medical; G-Bond Plus, GC Corp; Adper Easy Bond, 3M ESPE) in normal and caries-affected dentin. Methods and materials: Twenty molars with proximal caries were selected to TBS test, the mesial and distal enamel were trimmed to expose in one side de caries dentin and the other side the normal dentin. Then the caries was removed by manual excavator, helped with caries detector dye (Caries Check, Nippon Shika Yakuhin Co.), while in the normal dentin side, smear layer was manually standardized throughout #600 SiC paper for 60 s. The dentin hardness of caries affected was standardized with a manual hardness determination system (CARIOTESTER-SUK971, Saneime Corp). Then the both side of dentin (normal and caries-affected) were treated with one of adhesive systems tested (Clearfil Mega Bond, MTB-200, G- Bond Plus and Adper Easy Bond). It was utilized A2 shade resin composite (Clearfil AP-X, Kuraray Medical) to restore the nor- mal dentin side, while in the caries affected was utilized C4 shade. After 24 h stored in distilled water at 36 ◦ C, restored teeth was serially sectioned in X and Y directions, transver- sally to adhesive interfaces, resulting in a beam (1 mm 2 ) with two adhesive interfaces, one in normal dentin and the other one in caries-affected. For TBS test the beams were divided in two subgroups. The first one tested normal dentin inter- face and the other one the caries-affected dentin interface. The TBS was performed in a universal testing machine (EZ- Test, Shimazu) at crosshead speed of 1mm/min. Data were statistically analyzed by One-way ANOVA and Games – Howell post-hoc test, at 5% of significance level. Another 4 teeth with oclusal caries was selected for adhesive interface analysis in Fe-SEM (S-4000, Hitachi). Results: The predominant failure mode in all groups was adhesive; SEM images demonstrated thicker hybrid layer in caries affected dentin than normal dentin. Conclusion: The caries-affected condition of the dentine substrate influenced negatively the mean TBS for all adhe- sives tested, but statistically significant only for Clearfil Mega Bond and Clearfil MTB-200. Mean values of the TBS of the adhesive systems applied on normal dentin and caries affected, and standard deviation (±SD).