PNEC PERGAMON Psychoneuroendocrinology 28 (2003) 85 - 100 www.elsevier.com/locate/psyneuen Diazepam and progesterone produce sexually dimorphic actions on the rat EEG: role of the neonatal sexual differentiation process A. Fernindez-Guasti ",*, I.Y. del Rio Portilla b, E. Ugalde b, M. Corsi-Cabrera " Departumento de Farmacobiologia, CINVESTAV, Calzada de 10s Tenorios 235,Col. Granjas Coapa, Mexico City, 14330 D.F., Mexico Departamento de Psicologia, UNAM, Mexico City, Mexico Received 25 October 2001; received in revised form 24 January 2002; accepted 30 January 2002 Abstract This study was designed to analyse if the effect of diazepam or progesterone on the wakeful rat EEG vary according to sex and neonatal sexual differentiation. Diazepam or progesterone was administered to males, females, neonatally castrated males and neonatally virilised females. Comparisons of drug actions were made versus baseline values. Both compounds produced clear sexual dimorphic responses, males being more sensitive than females. In normal males and neonatally virilised females diazepam produced a clear increase in the absolute power of fast frequencies. In normal females and neonatally castrated males this anxiolytic only produced a moderate increase in the absolute power of the beta 2 band. Regardin, 0 inter- ' parietal correlation, diazepam produced an increase in males in the alpha, beta 1 and beta 2 bands, while in females this drug caused a reduction in the same bands. Neonatally castrated males or virilised females showed an intermediate pattern. These data suggest that sexual dimorphism in diazepam action depends upon neonatal sexual differentiation. Progesterone, in contrast with diazepam, produced effects on the EEG that were also sexually dimorphic but independent of the sexual differentiation process. This steroid in males induced an increase in the absolute power of the fast bands of the EEG (alpha, beta 1 and beta 2) accompanied by an increased interparietal correlation of the alpha band. In females, progesterone only reduced the interparietal correlation in the fast theta and alpha bands. Data show similarities between diazepam and progesterone effects on the EEG that are discussed in the light of the Corresponding author. Tel.: +52-54-83-28-56; fax: +52-54-83-28-63. E-mail addrcw: jfernand@mail.cinvestav.mx (A. Fernhndez-Guasti). 0306-4530/02/$ - see front matter O 2002 Elsevier Science Ltd. All rights reserved. PII: SO3O6-453O(O2)OOOll-2