High-resolution mapping of the barley Ryd3 locus controlling tolerance to BYDV Thomas Lu ¨pken • Nils Stein • Dragan Perovic • Antje Habekuß • Albrecht Serfling • Ilona Kra ¨mer • Urs Ha ¨hnel • Burkhard Steuernagel • Uwe Scholz • Ruvini Ariyadasa • Mihaela Martis • Klaus Mayer • Rients E. Niks • Nicholas C. Collins • Wolfgang Friedt • Frank Ordon Received: 12 May 2013 / Accepted: 23 September 2013 / Published online: 9 October 2013 Ó Springer Science+Business Media Dordrecht 2013 Abstract Barley yellow dwarf disease (BYD) is transmitted by aphids and is caused by different strains of Barley yellow dwarf virus (BYDV) and Cereal yellow dwarf virus (CYDV). Economically it is one of the most important diseases of cereals worldwide. Besides chemical control of the vector, growing of tolerant/resistant cultivars is an effective way of protecting crops against BYD. The Ryd3 gene in barley (Hordeum vulgare L.) confers tolerance to BYDV-PAV and BYDV-MAV and the locus was previously mapped on the short arm of barley chromosome 6H near the centromere. We applied a strategy for high-resolution mapping and marker saturation at the Ryd3 locus by exploiting recent genomic tools available in barley. In a population of 3,210 F2 plants, 14 tightly linked markers were identified, including 10 that co-segregated with Ryd3. The centromeric region where Ryd3 is located suffers suppressed recombination or reduced recom- bination rate, suggesting potential problems in achiev- ing (1) map-based cloning of Ryd3 and (2) marker selection of the resistance in breeding programmes without the introduction of undesirable traits via linkage drag. Electronic supplementary material The online version of this article (doi:10.1007/s11032-013-9966-1) contains supple- mentary material, which is available to authorized users. T. Lu ¨pken Á D. Perovic Á A. Habekuß Á A. Serfling Á I. Kra ¨mer Á F. Ordon (&) Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany e-mail: frank.ordon@jki.bund.de N. Stein Á U. Ha ¨hnel Á B. Steuernagel Á U. Scholz Á R. Ariyadasa Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Stadt Seeland (OT) Gatersleben, Germany M. Martis Á K. Mayer Munich Information Center for Protein Sequences/ Institute of Bioinformatics and Systems Biology (MIPS/ IBIS), Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, 85764 Neuherberg, Germany R. E. Niks Department of Plant Breeding, Graduate School of Experimental Plant Sciences, Wageningen University, P.O. Box 386, 6700 Wageningen, The Netherlands N. C. Collins Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA 5064, Australia W. Friedt Institute of Crop Science and Plant Breeding I, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany 123 Mol Breeding (2014) 33:477–488 DOI 10.1007/s11032-013-9966-1