6 In Vitro Techniques Edited by J. Robin Harris Nuclear components Protocol 6.1 Nucleosome assembly coupled to DNA repair synthesis using a human cell free system 204 Protocol 6.2 Single labelling of nascent DNA with halogenated thymidine analogues 210 Protocol 6.3 Double labelling of DNA with different halogenated thymidine analogues 214 Protocol 6.4 Simultaneous immunostaining of proteins and halogen-dU-substituted DNA 217 Protocol 6.5 Uncovering the nuclear matrix in cultured cells 220 Protocol 6.6 Nuclear matrix–lamin interactions: in vitro blot overlay assay 228 Protocol 6.7 Nuclear matrix–lamin interactions: in vitro nuclear reassembly assay 230 Protocol 6.8 Preparation of Xenopus laevis egg extracts and immunodepletion 234 Protocol 6.9 Nuclear assembly in vitro and immunofluorescence 237 Protocol 6.10 Nucleocytoplasmic transport measurements using isolated Xenopus oocyte nuclei 240 Protocol 6.11 Transport measurements in microarrays of nuclear envelope patches by optical single transporter recording 244 Cells and membrane systems Protocol 6.12 Cell permeabilization with Streptolysin O 248 Protocol 6.13 Nanocapsules: a new vehicle for intracellular delivery of drugs 250 Protocol 6.14 A rapid screen for determination of the protective role of antioxidant proteins in yeast 255 Protocol 6.15 In vitro assessment of neuronal apoptosis 259 Cell Biology Protocols. Edited by J. Robin Harris, John Graham, David Rickwood  2006 John Wiley & Sons, Ltd. ISBN: 0-470-84758-1