1 Running title: Analysis of C. elegans nuclear pore complexes Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans Peter Askjaer 1 , Vincent Galy 2 and Peter Meister 3 1 Andalusian Center for Developmental Biology (CABD), CSIC/JA/Universidad Pablo de Olavide, Carretera de Utrera, km 1, 41013 Seville, Spain. pask@upo.es 2 UMR 7622 CNRS-UPMC, Biologie du Développement, Université Pierre et Marie Curie 9 quai Saint-Bernard, 75252 PARIS Cédex 05, FRANCE. vincent.galy@snv.jussieu.fr 3 Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH-3012 Bern, Switzerland. peter.meister@izb.unibe.ch Keywords: Caenorhabditis elegans; CRISPR-Cas9; live imaging; npc; npp; nuclear pore complex; nucleocytoplasmic transport; nucleoporin; RNAi; super-resolution microscopy Abstract I. Introduction II. Methods A. Forward and reverse genetics B. Transgenesis C. Live imaging of embryos D. In vivo methods to evaluate structural and functional integrity of the NE E. Immunofluorescence and EM F. Interaction of Nups with chromatin III. Summary Acknowledgments References