1 Title 1 Regional and Temporal variations of Canine Leptospirosis across the United States, 2000- 2 2010 3 4 Authors 5 Hu Suk Lee, Michael Levine, Carolyn Guptill-Yoran, April Johnson, Peter von Kamecke, 6 George E. Moore 7 From the Departments of Comparative Pathobiology (Lee, Johnson, Moore), Statistics 8 (Levine), and Veterinary Clinical Sciences (Guptill-Yoran), Purdue University, West 9 Lafayette, IN 47907, United States; and IDEXX Laboratories (von Kamecke), Westbrook, 10 ME 04092, United States 11 12 Short title 13 Regional and temporal variations of canine leptospirosis 14 15 Keywords 16 Leptospira; dogs; MAT, seropositive; STL; seasonal cycle subseries plot 17 18 List of abbreviations used in the manuscript 19 MAT microscopic agglutination test, CI confidence interval, STL seasonal-trend 20 decomposition procedure based on loess, OR odds ratio, NE North-east, MW Mid-west, SC 21 South-central, CS California-southern west coast 22 23 Corresponding author and requests for reprints to: George E. Moore 24