Stabilization of glucose oxidase on glycation induced nano-fibrils: New insight in enzyme immobilization Sara Farahi 1 , Mehran Habibi-Rezaei* 1,2 , Azadeh Ebrahim-Habibi 3 , Amir Arasteh 4 , Nader Sheibani 5 , Ali Akbar Moosavi-Movahedi 6 1 School of Biology, College of Science, University of Tehran, Tehran, (IRAN) 2 Nanobiomedicine Center of Excellence, Nanoscience and Nanotechnology Research Center, University of Tehran, Tehran, (IRAN) 3 Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences Tehran, (IRAN) 4 Science and Research Branch, Islamic Azad University, Tehran, (IRAN) 5 Departments of Ophthalmology and Visual Sciences, and Pharmacology, University of Wisconsin School of Medicine and Public Health, Madison, WI, (USA) 6 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, (IRAN) E-mail: mhabibi@khayam.ut.ac.ir Received : 25 th December, 2013 ; Revised : 08 th March, 2014 ; Accepted : 18 th March, 2014 Abstract : Amyloids are interesting biomaterials with useful properties including high strength and resistance to degradation. These characteristics make amyloids suitable nano-structure candidates for bio-applications, including enzyme immobilization scaffold at nano-scale dimensions. Here, glycation induced bovine serum al- bumin (BSA) nano-fibrils were used as a scaffold for glucose oxidase (GOx) immobilization and the kinetic parameters optimum temperature and pH of the free and immobilized GOx were compared. The covalently bound GOx on BSA amyloid nano-fibers oxidized glu- cose to release hydrogen peroxide that offers a signifi- cant antimicrobial property against E. coli to the im- mobilized enzyme product. However, the enzyme s Original Article e re ), 32-40, 20 4) rint) 2320 1967 Online) 2320 1975 catalytic performance (k cat /K m ) was decreased due to the covalent immobilization on nanofibrils. In addition, a broadening and an alkaline shift in the temperature and pH profiles of the enzyme was observed. These changes were concomitant with improved stability of the GOx upon immobilization. Together our results show that BSA nano-fibrils provide a suitable nano- structure for immobilization of GOx with enhanced sta- bility and conserved catalytic activity.  Global Scientific Inc. Keywords : Amyloid; Catalytic nano-fibers; Glu- cose oxidase; Enzyme immobilization.