1 Asian J. Exp. Sci., Vol. 23, No. 3, 2009; 01-04 Biochemical and Molecular Aspects of DNA in Raw and Ripen Fruit and Vegetables Ujjwala Krothapalli, Lakshmi Sai Bharani Nuthi and Vemuri Praveen Kumar* Department of Biotechnology, Koneru Lakshmaiah College of Engineering, Vaddeswaram, Guntur District-522502 (A.P.); India. Abstract : Vegetables, fruits, plant and animal products contain DNA in various amounts. DNA, the carrier of genetic information, the recipes or blueprints of an organism, is made up of four different chemical units. These can be digested and used just like any other nutrient in our food. Usually DNA is degraded during cooking, but even if DNA is eaten uncooked, like in the case of apples, a tomato, or a salad, the DNA is degraded rapidly in our stomach. Eating DNA should not be a concern: even mother’s milk contains high amounts of DNA, because newborns have a higher need for them. Studies were carried out to give us an idea of the amount of DNA we eat and some of its physical properties. Key words : Climate disasters, Resource wars, Food shortages, Sovereignty, Overconsumption, Greenhouse gas emissions, Environmental mitigation. Introduction Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms and some viruses. DNA was first isolated in 1869 by Friedrich Miescher. The main role of DNA molecules is the long-term storage of information. DNA is the hereditary material in humans and almost all other organisms. The order, or sequence, of the nucleotide bases present in the DNA determines the information available for building and maintaining an organism. It is this sequence that is referred to as the genetic code, and it is sometimes called the code of life or blueprint of life. The organism which is rich in nucleic acids alternatively has a bigger chance to synthesize proteins through central dogma process. Thus the work indicates the choice of fruit or vegetable in raw or ripen condition for more consumption of nucleic acids into the body. DNA is isolated by placing the cells in a tube containing a special solution and mechanically or chemically breaking the cells open. This causes the cell to release its contents into the solution which may contain enzymes, chemicals, and salts. Enzymes are used to chew up the proteins; chemicals to destroy any RNA present and salts to help pull the DNA out of solution. The DNA will exist in long strands that form a mucous-like glob. The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. Materials and Methods Preparation of smoothie The various raw and ripen fruits and vegetables like Cucumber, Tomato, Banana and Papaya was obtained form Vijayawada market. They are cleaned thoroughly with distilled water for the removal of contaminants or dust which may degrade the DNA. The surface layer of the fruits and vegetables are * Corresponding author : Vemuri Praveen Kumar, 2F, Golden Towers, Near Durgamahal, Patamata, Vijayawada-10 (A.P.); India; Cell : (0)9849629496; E-mail : vemuripraveen@gmail.com