http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, Early Online: 1–2 ! 2015 Informa UK Ltd. DOI: 10.3109/19401736.2015.1018207 MITOGENOME ANNOUNCEMENT The complete mitogenome of the endangered white-clawed freshwater crayfish Austropotamobius pallipes (Lereboullet, 1858) (Crustacea: Decapoda: Astacidae) Frederic Grandjean 1 , Mun Hua Tan 2,3 , Huan You Gan 2,3 , Han Ming Gan 2,3 , and Christopher M. Austin 2,3 1 Laboratoire Ecologie et Biologie des Interactions, UMR CNRS 7267 Equipe Ecologie Evolution Symbiose, Poitiers Cedex, France, 2 School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor, Malaysia, and 3 Monash University Malaysia Genomics Facility, Jalan Lagoon Selatan, Bandar Sunway, Selangor, Malaysia Abstract The Austropotamobius pallipes complete mitogenome has been recovered using Next-Gen sequencing. Our sample of A. pallipes has a mitogenome of 15,679 base pairs (68.44% A + T content) made up of 13 protein-coding genes, 2 ribosomal subunit genes, 22 transfer RNAs, and a 877 bp non-coding AT-rich region. This is the first mitogenome sequenced for a crayfish from the family Astacidae and the 4 th for northern hemisphere genera. Keywords Astacidea, conservation, molecular resources History Received 11 January 2015 Accepted 24 January 2015 Published online 4 March 2015 Europe has a relatively impoverished freshwater crayfish fauna compared to north America and Australasia but includes several species of economic and ecological significance and, unfortu- nately increasingly of conservation concern (Huner, 1994; Holdich et al., 2009). All native species are placed in two genera Astacus and Austropotamobius, which contains three and two currently recognized species respectively (Souty-Grosset et al., 2006), although cryptic diversity has been found in several species on the basis of molecular genetic studies (Grandjean et al., 2002; Maguire et al., 2014; Pedraza-Lara et al., 2010). Unfortunately, the number of alien crayfish species established in European freshwaters now exceeds native crayfish diversity (Holdich et al., 2009) thus representing a significant threat to native species. One of the most threatened species of European freshwater crayfish is Austropotamobius pallipes which most likely is a species complex comprising at least two genetically distinct species A. pallipes in the north and a southern form, A. italicus, occurring largely restricted to Spain, Italy and Balkans (Grandjean et al., 2002). However, despite its relatively large distribution, the species is assessed by the IUCN as endangered Correspondence: Han Ming Gan, School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 46150 Petaling Jaya, Selangor, Malaysia. E-mail: gan.han.ming@monash.edu Table 1. Annotation of the complete mitochondrial genome of A. pallipes. Gene Strand Position Length (bp) Intergenic nucleotides (bp) Start Codon Stop Codon Anticodon cox1 + 1–1535 1535 0 ACG TTA trnL2(taa) + 1536–1598 63 0 UAA cox2 + 1542–2285 744 57 ATG TAA trnK(ttt) + 2287–2350 64 1 UUU trnD(gtc) + 2352–2420 69 1 GUA atp8 + 2430–2579 150 9 ATA TAG atp6 + 2573–3247 675 7 ATG TAA cox3 + 3247–4035 789 1 ATG TAA trnG(tcc) + 4034–4095 62 2 UCC nad3 + 4096–4449 354 0 ATT TAA trnA(tgc) + 4454–4514 61 4 UGC trnR(tcg) + 4514–4576 63 1 UCG trnE(ttc) + 4577–4640 64 0 UUG putative control region + 4641–5517 877 0 trnQ(ttg) – 5518–5586 69 0 UUG trnS1(tct)  5593–5659 67 6 UCU (continued ) Mitochondrial DNA Downloaded from informahealthcare.com by 101.103.26.65 on 03/04/15 For personal use only.