Mesopotamia Environmental Journal ISSN 2410-2598 Mesop. environ. j. 2016, Vol.2, No.2:75-86. www.bumej.com 75 Effect of phosphorus concentration and light intensity on protein content of microalga Chlorella vulgaris Maysam Alaa Baiee Jasim M. Salman Department of Biology, College of Science, University of Babylon, Iraq. Corresponding author: maysam.baiee@yahoo.com To cite this article: Baiee, M. A. and Salman J. M. Effect of phosphorus concentration and light intensity on protein content of microalga Chlorella vulgaris. Mesop. environ. j., 2016, Vol. 2, No.2, pp. 75-86. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. Abstract The exploitation of microalgae as a protein source has led to increased interest in the use of microalgae in health food production and source of animal feed. Three light intensities (125, 268 and 300 μE\m²\sec) and four phosphorous concentrations (0, 2, 4, 6, g\l) were used to study their effect on the growth and amount of protein content in Chlorella vulgaris alga. The growth curve of the studied alga was different among the treatments. The stationary phase began at the days 13, 12 and 9 for treatments 125, 268 and 300 (μE\m²\sec) respectively, while it was identified as day 14, 12, 10 and 6 in treatments 6, 4, 2 and 0 g\l of phosphorous respectively. The highest K value was 0.15 at the 268 μE\m²\sec (control), while the lowest K value was 0.08 at 125 μE\m²\sec. The shortest doubling time (G) was 2 days at control (268 μE\m²\sec) treatment. When used different phosphorous concentrations, The highest K value was 0.16 at 0 g\l treatment, while the lower K value was 0.1 at 6 g\l treatment. The shortest doubling time (G) was 1.8 day in treatment 0 g\l. Protein content of C. vulgaris increased from 39.46% to 57.51% at 125 μE\m²\sec to 300 μE\m²\sec light intensity. When used different phosphorus concentrations, protein content increased from 51.17% at control treatment (4 g\l) to 75.56% at 6 g\l treatment. Keywords; Protein, Chlorella vulgaris, light intensity, phosphorous concentration Introduction Algae have been used in animal and human diets since very early times. The lipids and starch fraction in algae can be used for bio- oil production, the residual algae cake, which is rich in proteins, is