357 Review www.expert-reviews.com ISSN 1473-7140 © 2009 Expert Reviews Ltd 10.1586/14737140.9.3.357 Anthracyclines are a highly efficacious treatment for hematological malignancies, including acute myeloid leukemia, acute lymphoblastic leukemia, multiple myeloma, Hodgkin’s disease and non- Hodgkin’s lymphoma (NHL). Doxorubicin- based combination chemotherapy continues to be a cornerstone of chemotherapy for NHL. The cyclophosphamide, doxorubicin, vinc- ristine and prednisolone (CHOP) regimen is the gold-standard treatment for patients with aggressive NHL [1] . This first-generation com- bination chemotherapy is associated with a complete remission (CR) rate of approximately 50%, with 30% of patients being alive and dis- ease free 5 years after chemotherapy [1] . Many attempts have been made to improve the clini- cal outcome with second- and third-generation schedules, without any significant improvement in terms of CR rate, disease-free survival (DFS) or overall survival (OS) [2–4] . The addition of rituximab (MabThera ® , Roche, Switzerland; Rituxan ® , Genentech-Biogen IDEC, USA), a monoclonal antibody against the CD20 antigen expressed on the vast majority of B-cell lymphomas, to the CHOP regimen has been shown to improve outcome in elderly patients with advanced disease, without any significant increase in toxicity [5] . Subsequent studies have also confirmed the efficacy of this approach in younger patients [6] . However, multiple relapses are common for patients with NHL, resulting in second-, third-, fourth- or possibly even fifth-line treat- ment during a period of 12–15 years. The main problem is that salvage therapy may well be with an anthracycline-based regimen, which could lead to cumulative doses of doxorubicin of more than 450 mg/m 2 . Cardiotoxicity is still a key medical concern because the cumulative toxicity of anthracyclines is dose limiting and irreversible [7–9] . Liposomal formulations have been developed with the aim of improving the therapeutic index of doxorubicin by reducing the drug’s cardio- toxicity but maintaining its anti-tumor effi- cacy. Two liposomal formulations are currently Giuseppe Visani and Alessandro Isidori Author for correspondence Hematology and Hematopoietic Stem Cell Transplant Center, San Salvatore Hospital, Via Lombroso 1, 61100 Pesaro, Italy Tel.: +39 072 136 4039 Fax: +39 072 136 4036 g.visani@ospedalesansalvatore.it Anthracyclines, including doxorubicin, are widely used in the treatment of B-cell non-Hodgkin’s lymphoma (NHL). However, their clinical potential is limited by their cardiotoxic adverse effects, which include cardiomyopathy and congestive heart failure. Anthracycline-induced cardiotoxicity is cumulative and irreversible. Liposomal doxorubicin has been developed with the aim of improving the therapeutic index of doxorubicin by reducing the drug’s cardiotoxicity. Nevertheless, liposomal conjugation of doxorubicin results in preferential distribution of the drug in the tumor compared with normal tissue, maintaining its anti-tumor efficacy. Nonpegylated liposomal doxorubicin produced a promising response rate when substituted for conventional doxorubicin in the cyclophosphamide, doxorubicin, vincristine and prednisolone regimen in the treatment of patients with NHL either at diagnosis or at relapse. The ability of nonpegylated liposomal doxorubicin to overcome excessive drug efflux due to P-glycoprotein (MDR-1) overexpression in NHL might confer on this drug a curative potential for patients with a bad prognosis (e.g., MDR-1 overexpressing, the elderly or frail). KEYWORDS:฀anthracycline฀•฀B-cell฀non-Hodgkin’s฀lymphoma฀•฀cardiotoxicity฀•฀eficacy฀•฀nonpegylated฀liposomal฀ doxorubicin฀•฀safety Nonpegylated liposomal doxorubicin in the treatment of B-cell non-Hodgkin’s lymphoma: where we stand Expert Rev. Anticancer. Ther. 9(3), 357–363 (2009) For reprint orders, please contact reprints@expert-reviews.com