Research Article Libidibia ferrea Mature Seeds Promote Antinociceptive Effect by Peripheral and Central Pathway: Possible Involvement of Opioid and Cholinergic Receptors Luis Armando Sawada, 1,2 Vanessa Sâmia da Conçeição Monteiro, 2 Guilherme Rodrigues Rabelo, 3 Germana Bueno Dias, 3 Maura Da Cunha, 3 José Luiz Martins do Nascimento, 1 and Gilmara de Nazareth Tavares Bastos 2 1 Laborat´ orio de Neuroqu´ ımica Molecular e Celular, Instituto de Ciˆ encias Biol´ ogicas, Universidade Federal do Par´ a (UFPA), Rua Augusto Correa s/n, Guam´ a, 66075-900 Bel´ em, PA, Brazil 2 Laborat´ orio de Neuroinlamac ¸˜ ao, Instituto de Ciˆ encias Biol´ ogicas, Universidade Federal do Par´ a (UFPA), Rua Augusto Correa s/n, Guam´ a, 66075-900 Bel´ em, PA, Brazil 3 Laborat´ orio de Biologia Celular e Tecidual, Centro de Biociˆ encias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Avenida Alberto Lamego 2000, Parque Calif´ ornia, 28013-602 Campos dos Goytacazes, RJ, Brazil Correspondence should be addressed to Gilmara de Nazareth Tavares Bastos; gbastos2005@hotmail.com Received 28 November 2013; Revised 4 February 2014; Accepted 2 March 2014; Published 22 April 2014 Academic Editor: Eiichi Kumamoto Copyright © 2014 Luis Armando Sawada et al. his is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Libidibia ferrea (LF) is a medicinal plant that holds many pharmacological properties. We evaluated the antinociceptive efect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms. Histochemical tests and Gas chromatography of the LPLF were performed to characterize its fatty acids. Acetic acid-induced abdominal constriction, formalin-induced pain, and hot-plate test in mice were employed in the study. In all experiments, aqueous extract or LPLF was administered systemically at the doses of 1, 5, and 10mg/kg. LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive efect in all tests indicating both peripheral anti-inlammatory and central analgesia properties. Also, the use of atropine (5 mg/kg), naloxone (5 mg/kg) in the abdominal writhing test was able to reverse the antinociceptive efect of the LPLF, indicating that at least one of LF lipids components is responsible for the dose related antinociceptive action in chemical and thermal models of nociception in mice. Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such efect in previous studies. 1. Introduction Libidibia ferrea (LF) is a plant used in folk medicine for therapeutical purposes. LF is a tree species from the Legu- minosae family, one of the largest dicotyledon families with approximately 650 genus, that gathers more than 18,000 species. It is found along all tropical regions in Brazil but manly in the north and northeast areas [1]. Previous studies identiied many pharmacological prop- erties of LF, which explain its common use in folk medicine, for treatment of wounds, bruises, chronic cough, and asthma [2]. he fruits can be used to treat diabetes and to prevent cancer. Its extract proved in in vivo tests wound healing [3]. Roots are utilized as antipyretic and treatment of diarrhea. LF has also demonstrated anticancerogenic, antioxidant, wound repair, and DNA protection properties [4, 5]. Besides, the bark is used for enterocolitis, diabetes, and rheumatism treatment [6]. hus, in the present study, we identiied the main storage substances and their accumulation sites in Libidibia ferrea Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 508725, 10 pages http://dx.doi.org/10.1155/2014/508725