Asian Journal of Chemistry Vol. 19, No. 3 (2007), 2438-2444 Effect of Iron Applications on Fe, Zn, Cu and Mn Compositions of Grapevine Leaves GÜLTEKIN ÖZDEMIR* and SEMIH TANGOLAR Department of Horticulture, Faculty of Agriculture, Dicle University 21280, Diyarbakir, Turkey Fax: (90)(412)2488153; Tel: (90)(532)5450720; E-mail: gozdemir@gmail.com In present study, different grapevine genotypes having dif- ferent resistance levels to CaCO3 were used as plant material. Genotypes were grown in pots including CaCO3 concentra- tions of 10, 30 and 50 %. For each medium, 4 different soil with Fe applications were performed. These are: i) 20 ppm Fe (as FeSO4) + farmyard manure (100 g/pot/5 kg soil), ii) 20 ppm Fe (as Fe-EDDHA) iii) 20 ppm Fe (as FeSO4) + citric acid (as 10 % percentage of applied FeSO4), iv) control (soil without Fe). In order to determine the effects of applications on micro element concentrations of leaves were examined. As a result of the study, Fe, Zn, Cu and Mn concentrations of all tested genotypes were decreased with increasing levels of CaCO3. Applications of Fe-EDDHA and FeSO4 + citric acid showed better results when all applications compared in respect of leaves Fe, Zn, Cu and Mn concentrations. The high- est Fe, Zn, Cu and Mn concentrations were determined in Yalova incisi genotype. Key Words: Grapevine, Iron fertilizer, Calcareous soil, Micro elements, Vitis species. INTRODUCTION Iron is one of the most important elements in mineral nutrition of grapes. Its relatively high abundance in the earth's cultivated calcareous soils, grape- vine Fe acquisition is often impaired, a fact resulting in severe crop losses. Among the soil properties that impair Fe nutrition problems, calcium carbonate, whose presence is wide-spread on 30 % of total land area 1 plays a mojor role 2 . Many agricultural crops, grown in calcerous soils, suffer from micro element deficiency in leaves. Vitis species differ in their degree of suscep- tibility to calcerous soils 3-5 . Vitis vinifera L. is ranked as calcerous tolerant, but with a range of variation within the varities 2 . Several reviews on iron nutrition of higher plants are available, cover- ing, among others, iron availability for root uptake 6,7 , iron nutrition in calcareous soils 1,8 , plant susceptibility to iron deficiency and plant adapta- tion mechanisms 9-13 , prevention and correction of chlorosis 1,14-16 . Bavaresco 17