FOR THE RECORD Ga Liao, 1 Ph.D.; Tingting Liu, 2 Ph.D.; Binwu Ying, 3,4 Ph.D.; Lei Sun, 5 M.D.; Yi Zou, 1 Ph.D.; Zhengrong Wang, 1 Ph.D.; Hong Fan, 3,4 Ph.D.; and Fuqiang Wen, 3 M.D. Population Genetic Study of 15 STR Loci in a Chinese Population POPULATION: Chinese (n = 501). KEYWORDS: forensic science, Han ethnic group, Guangzhou City, Guangdong Province, China, DNA typing, short tandem repeats, poly- merase chain reaction, population genetics, D3S1358, THO1, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, VWA, D8S1179, TPOX, FGA Blood samples were collected from 501 unrelated people of the Han ethnic group in Guangzhou, China. DNA was extracted using Chelex method (1). Individual DNA samples were amplified for 16 STR loci (including Amelogenin) by PCR using Powerplex 16 TM kit (Promega Corporation, Madison, WI) under the conditions rec- ommended by the manufacturer in a reaction volume of 12.5 lL in PE 9600 thermal cycler (Perkin-Elmer, Foster City, CA) (2,3). The PCR product of 1 lL was mixed with 0.3 lL internal LIZ size stan- dard (GS-500 LIZ; Applied Biosystems, Foster City, CA) and 8.7 lL Hi-Di formamide (Applied Biosystems), and electrophoresed in ABI 3100 Genetic Analyzer (Applied Biosystems) using the rec- ommended protocol from the kit. The results were analyzed by Data Collection (Version 1.1), GeneScan (Version 3.7), and Genotyper (Version 3.6) softwares (Applied Biosystems) (Tables 1 and 2). Evaluation of Hardy–Weinberg expectations was carried out using the chi-squared test; the power of discrimination (PD), the probability of paternity exclusion (PE), the polymorphic informa- tion content (PIC), and the observed heterozygosity (Ho) were cal- culated using Powerstate of the Version 1.2 package (4,5). The complete data (also including some statistical parameters) are available to any interested researcher upon request. References 1. Walsh BS, Petzger DA, Higuchi R. Chelex-100 as medium for simple extraction of DNA for PCR-based typing from forensic material. Biotech- niques 1991;10:506–10. 2. Promega Corporation. PowerPlexR 16 system kit, technical manual, http://www.promega.com. Accessed March 2006. 3. Krenke BE, Tereba A, Anderson SJ, Buell E, Culhan S, Finis CJ, et al. Validation of a 16-locus fluorescent multiplex system. J Forensic Sci 2002;47:773–85. 4. Tereba A. Tools for analysis of population statistics: profiles in DNA, Vol. 2, 1999; 203. Available at http://www.promega.com/geneticidentity/. Accessed March 2006. 5. Ying BW, Wei YG, Sun XM, Liu TT, Hou YP. STR data for the AmpFlSTR profiler plus from western China. J Forensic Sci 2005; 50(3):716–7. Additional information and reprint requests: Fuqiang Wen, M.D. Department of Respiratory Medicine Sichuan University (West China University of Medical Sciences) Chengdu 610041, Sichuan China E-mail: fwen78@yahoo.com ⁄ bioion@gmail.com 1 Biomedical Engineering Department, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. 2 Department of Orthodontics, West China College of Stomatology, Sichu- an University, Chengdu 610041, Sichuan, China. 3 Department of Respiratory Medicine, West China Hospital, Sichuan Uni- versity, Chengdu 610041, Sichuan, China. 4 Department of Laboratory Medicine, West China Hospital, Sichuan Uni- versity, Chengdu 610041, Sichuan, China. 5 Department of Paediatrics, The 2nd West China Hospital, Sichuan Uni- versity, Chengdu 610041, Sichuan, China. J Forensic Sci, January 2008, Vol. 53, No. 1 doi: 10.1111/j.1556-4029.2007.00632.x Available online at: www.blackwell-synergy.com 252 Ó 2008 American Academy of Forensic Sciences