Original Article EVALUATION OF IN VITROANTICANCER ACTIVITY AND GC-MS ANALYSIS FROM LEAF SOPHORA INTERRUPTA BEDD PARDHASARADHI MATHI 1 , KUMAR NIKHIL 2 , SNEHASISH DAS 2 , PARTHA ROY 2 , VENKATA RAMAN BOKKA 3 , MAHENDRAN BOTLAGUNTA 1* 1 Biomedical research Laboratory, Department of Biotechnology, K L E F University, Green fields, Vaddeswaram 522502, Guntur District, Andhra Pradesh, India, 2 Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India, 3 Received: 29 Jan 2015 Revised and Accepted: 28 Feb 2015 ABSTRACT Objective: Sophora interrupta Bedd (Fabaceae) is one of the well-known medicinal herb used in folk medicine for the treatment of cancer and inflammatory associated diseases. In this paper we aimed to evaluate the antioxidant and anti-cancer, properties of aqueous, methanol and n-hexane leaf extracts. Methods: The leaf extracts were analyzed for antioxidant activity using DPPH free radical scavenging assay and anticancer activity by measuring the cell viability using MCF-7 and PC-3 cancer cell lines. GC-MS analysis was performed to identify anti-cancer compounds present in the active leaf extract. Results: The polar solvent extracts showed good antioxidant at 500 μg/ml as related to non-polar solvents. Moreover, methanol extract exhibited highest percentage of cell death, in both MCF-7 (IC Department of Basic Sciences-Chemistry, Madanapalle Institute of Technology and Science (MITS), Madanapalle 517325, Chittoor District, Andhra Pradesh, India Email: bmnchowdary@gmail.com 50 500 μg/ml) and PC-3 cells (IC 50 Conclusion: Over all, it suggests that leaf methanol extract contain anticancer compounds, which are evident from our GC-MS analysis. 1000 μg/ml), which is also evident from morphological observations, acridine orange and ethidium bromide dye exclusion assays. Keywords: Sophora interrupta Bedd, Leaves, Methanol, Antioxidant, GC-MS. INTRODUCTION Medicinal plants in a form of vegetable or in a form of food ingredients have a great importance in our daily life to maintain a balanced diet. Apart from food they also play a very important role in uptake of vitamins and minerals. For example, drinking of tulasi leaf soaked water, has shown to restore the iron absorption in anemic patients [1]. Along the lines, Terminalia tomentosa bark juice has shown to cure several cardiovascular diseases and therefore, it was considered as a cardiac tonic. Indeed, natural compounds and their derivatives represent more than 50% of all the drugs in clinical use for today’s world. During the last 4 decades, a dozen of potent drugs have been gained from flowering plants; one example is that of Paclitaxel, a drug template derived from the bark of Taxus brevifolia species used to treat a number of cancers [2]. Other examples include anticancer agents derived from Catharanthus roseus as well as laxative agents from Cassia species [3]. Therefore, exploration of medicinal plants and their importance to human and animal health wellbeing is an ongoing research. Sophora interrupta Bedd (S. interrupta) was commonly known as Edwariamadarasapatna and distributed in high altitude region of holy tirumala hills, Andhra Pradesh, India. Many years, this plant was employed in Ayurveda for the treatment of non-communicable diseases like cancer and cardiovascular diseases. Several active compounds, such as O-prenylated flavonol i. e; 3′ , 4′ -dimethoxy-7-(γ, γ- dimethylallyloxy) flavonol a novel compound. 2′ -hydroxy-3, 4- dimethoxychalcone was a well-known natural phenol. Biochanin A was an isoflavone and well explored. Kaempferol-3-O-β-D-glucopyranoside was also a natural flavonol, were isolated from root methanol extract of S. interrupta [4]. In a separate experiment similar extract significantly suppressed the tumor volume and enhanced the survival time of DAL bearing mice without altering the total hemoglobin content, RBC and WBC counts [5]. In our previous study, root EtOAc significantly reported anticancer activity against cancer cells and insilico work explainedKaempferol-3-O-b-D-glucopyranoside, a secondary metabolite of S. interrupta root formed 6 hydrogen bond interactions with Arg 202, Gln 207, Gly 227, Gly 229, Thr 231 and Ala 232 amino acids of human DEAD box RNA helicase, DDX3 protein suggesting that the root methanol extract contain anti-neoplastic compounds [6]. Despite these reports, no studies have been made to identify the active constituents present in this plant. In this paper we report, the comparative phytochemical composition, antioxidant activity of various solvent extracts of S. interrupta leaf, anti-cancer activity of leaf methanol extracts (MeOH) using MCF-7 and PC-3 cancer cell lines. Of the three extracts MeOH extract was shown significant anticancer and the novelty in this article was addressed by further exploring the compounds present in it by GC-MS analysis. MATERIALS AND METHODS Materials Human breast adenocarcinoma cancer cell line (MCF-7), Prostate adenocarcinoma cell line (PC-3) and Human embryonic kidney cell line (HEK293) was obtained from theNational Center for Cell Science (NCCS), Pune, India. Cell culture reagents were acquired from GIBCO (Invitrogen USA). Streptomycin, Penicillin, MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5- diphenyl-2H-tetrazolium bromide), DMSO cell culture grade purchased from Hi-Media, Mumbai, India. The shrub S. interrupta was collected from Tirumala hilltopin the noon time. Forest area in the location of Latitude13.667790 & Longitude 79.345880 locations, and tender leaves was collected. The plant was taxonomically pointed out by Dr. A. Prasada Rao, Senior Botanist in K LE F University, Vijayawada, Andhra Pradesh, India. Avoucher specimen has deposited, in the department (voucher number KLU 1211) for further use. Methodologies Phytochemical analysis The presence of the phytoconstituents such as terpenoids, tannins, flavonoids, alkaloids, saponins, glycosides and anthraquinones was carried out based on standard protocols [7]. Measuring antioxidants by DPPH free radical Radical scavenging potentials of n-hexane, MeOH and Aqueous(AQ) extracts were determined against free radical DPPH from standard protocol [8]. Various concentrations of extract (100, 200, 300, 400, International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 7, Issue 5, 2015 Innovare Academic Sciences