Volume 3 • Issue 4 • 1000156
Clin Microbial
ISSN: 2327-5073 CMO, an open access journal
Research Article Open Access
Moghaddam et al., Clin Microbial 2014, 3:4
http://dx.doi.org/10.4172/2327-5073.1000156
Review Article Open Access
Clinical Microbiology: Open Access
Quorum Sensing in Bacteria and a Glance on Pseudomonas aeruginosa
Mehrdad Moosazadeh Moghaddam
1,2
*, Samaneh Khodi
1
and Ali Mirhosseini
1
1
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
2
National Institute of Genetic engineering and Biotechnology, Tehran, Iran
Keywords: Quorum sensing; Pseudomonas aeruginosa; Autoinducer;
Cell-to-Cell signaling
Introduction
Pseudomonas aeruginosa is an opportunistic pathogen that
primarily infects patients with immune deiciency such as neutropenia.
Generally, the pathogenesis of Pseudomonas is complex and
multifactorial, since the bacterium is both invasive and toxigenic, can
cause a range of infections in humans (Table 1) [1-3]. Pseudomonas
clearance is oten challenging due to immunodeiciency in patients
with infection, multidrug resistancy and sometimes β-lactamase
production and the inactivation of third-generation cephalosporin
[4,5]. P. aeruginosa produces a variety of cellular structures and
products involved in the ability to cause disease through enforcing the
adhesion, protecting the phagocytosis, modifying the immune response
or destroying the host tissue. Generally, the efective factors involved
in the development of infection by this bacterium can be divided into
two categories: Extracellular virulence factors and structural virulence
factors. Extracellular factors include pigments that are active in Fe
absorption, prevent the growth of other bacterial species and degrade
factors including protease, hemolysins and toxins (Figure 1) [6,7].
Major structural factors in this bacterium are pili, lagellum
and LPS. In addition to these factors, P. aeruginosa has also other
capabilities that enhance bacteria pathogenesis, including a broad-
spectrum Beta-Lactamase (ESBLs) of class A-B and D that is one of
*Corresponding author: Mehrdad Moosazadeh Moghaddam, Applied Biotechnology
Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran, P.O. Box
19395-5487, Tel: +982182482549; E-mail: mm.genetics@gmail.com
Received April 25, 2014; Accepted June 13, 2014; Published June 20, 2014
Citation: Moghaddam MM, Khodi S, Mirhosseini A (2014) Quorum Sensing
in Bacteria and a Glance on Pseudomonas aeruginosa. Clin Microbial 3: 156.
doi:10.4172/2327-5073.1000156
Copyright: © 2014 Moghaddam MM, et al. This is an open-access article
distributed under the terms of the Creative Commons Attribution License, which
permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
Abstract
Quorum sensing is a key behaviour-coordination mechanism employed by many bacteria to
regulate gene expression in accordance with population density through the use of signal molecules, known as
autoinducers. The quorum sensing is used by Bacteria populations to communicate and coordinate their group
interactions, which is typically applied by pathogens in infection processes. Generally, the quorum sensing pathways
in bacteria are composed of several main parts, including bacteria populations, signal molecules, protein activators
and target genes. In this system, the bacteria secrete the signal molecules into the environment and the concentration
increases gradually as the bacteria population grows. In a certain concentration threshold, the molecules become
detectable to the bacteria populations, and then activate target genes that regulate various behaviours, such as
virulence factors. In Pseudomonas aeruginosa, the expression of many virulence factors appears to be controlled
by quorum sensing. So, according to the role of this mechanism in the regulation and production of many virulence
factors, the function of quorum sensing is required for Pseudomonas aeruginosa to cause disease and infection. In
this article, we discussed the quorum sensing mechanism in Gram negative and positive bacteria with a closer look
at the Pseudomonas aeruginosa.
the major causes of high bacterial resistance to most commonly used
antibiotics; an exopolysaccharid known as bioilm matrix and secreted
Figure 1: Virulence factors in Pseudomonas aeruginosa.
Infection Related Risk Factors
Pneumonia
Old age, Chronic obstructive pulmonary disease, Cystic
ibrosis, Mechanical ventilation
Bacteremia Immunocompromised
Urinary tract Use of urinary catheter
Soft tissue Burns, Open wounds, Postsurgery
Diaberic foot Diabetes, Impaired microvascular circulation
Keratitis
Extended contact lens wear,
Contaminated contact lens solution
Otitis externa Tissue injury, Water blockage in ear canal
Otitis media folliculitis Improperly cleaned hot tubs
Table 1: Common infections causing by Pseudomonas aeruginosa [3].