Abstracts / Toxicology Letters 196S (2010) S37–S351 S127 To grant their safety some indicators, both chemical and microbio- logical, are measured to assure the quality of water. However, these waters can be a mix of substances with potential interactions or can include chemicals that may not be detected in individual parameter analysis. The aim of this project is design a battery of tests evaluating potential toxic effects at different levels of samples coming from different treatment plants. This work represents a first approach where some tests have been conducted to evaluate systemic tox- icity, reproductive toxicity and genotoxicity both at health and environmental levels. The tests have been performed according to guidelines approved by different regulatory entities. At environ- mental level we have evaluated the effect of the samples in plant germination, in reproduction and genotoxicity of earthworms and in embryonic development on fish following the protocols of the OECD guidelines. At health level cell viability and transforming ability as a marker of carcinogenicity have been evaluated. Results show that the assays used are adequate for the aim of the project and seem to be suitable to its application as a complement to the traditional determinations. Acknowledgements: This study was supported by the SOSTAQUA Project (led by Aguas de Barcelona and funded by CDTI in the frame- work of the Ingenio 2010 Programe under the CENIT call). Keywords: Wastewater; Systemic toxicity; Genotoxicity; Carcino- genicity doi:10.1016/j.toxlet.2010.03.443 P108-043 Effect of a waste incinerator on hematology values in two species of wild rodents in Northwestern Spain M.T. Gómez Mora, J. De Lapuente, J. Serret Salse, M. Borras Suarez Parc Cientific de Barcelona, Spain Within a study of ecosurveillance, wild rodents of two species, Apodemus sylvaticus and Mus spretus, have been captured in three areas in the surroundings of a waste incinerator (Z0 and Z1, 1 km to the South and 2 km to the NW of the incinerator, respectively; Z2, 5 km to the East) and, as a control, in a matched, clean zone, 10 km apart from the pollution focus (C). We present here the compari- son of hematological values among the different locations in both species. For Mus spretus, the white blood cells count (WBC) was signif- icantly decreased in correlation with the distance to the pollution focus, from normal values in the control zone (5.29 ± 1.59) to min- imal ones in Z0 (4.32 ± 1.10), with intermediate values in Z2 and Z1 (4.97 ± 1.84 and 4.81 ± 0.97). Lymphocytes, taken alone, also showed its minimal level in Z0 (2.46 ± 0.74) with respect to C (3.99 ± 1.11). Although not significant, a slight trend to decrease of RBC values with the distance to the pollution focus was also observed. In the case of Apodemus sylvaticus, the number of captures at C was too small, and the comparisons have been only possible among the polluted locations. However, there are no evident differences among locations in any parameters. In this scenario and with the parameters studied, Mus spre- tus has shown to be more sensitive than Apodemus sylvaticus to pollution effects. Our results suggest that, in this case, in Mus spretus there is an effect on hematology parameters, with a trend to immunodepres- sion and erytrocitosis, possibly correlated to a pollution gradient going along with distance from the focus. In that sense, hema- tological values in certain species of wild rodents prove to be a useful indicator of health in populations exposed to environmental pollution. doi:10.1016/j.toxlet.2010.03.444 P108-044 Effects of lead on bone biomineralization in free-living wild boar (Sus scrofa) from a polluted mining area J. Rodríguez-Estival 1 , P. Álvarez-Lloret 2 , L. Monsalve-González 1 , A. Rodríguez-Navarro 2 , R. Mateo 1 1 Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Spain, 2 Universidad de Granada, Facultad de Ciencias, Spain Mining and smelting activities have contributed largely to increase the concentrations of heavy metals in the ecosystems, threaten- ing natural habitats and the health of wildlife. Wild ungulates are exposed to Pb when feeding on polluted mining areas or due to the ingestion of polluted soil. Pb mainly accumulates in bone tis- sue, where it may disturb several metabolic processes related to bone tissue turnover and remodelling, affecting biochemical and mechanical properties. In this study we have evaluated the toxic effects of Pb exposure on bone biomineralization in free-living wild boar (Sus scrofa) from a lead mining area in Spain, using Fourier transform infrared spectrometry (FTIR) and X-ray diffraction (XRD) techniques. Wild boar from the mining area had higher Pb levels in bone than animals from control areas (mean (95%CI) in g/g d.w.: 6.433 (3.459–11.965) vs. 1.151 (0.873–1.517)). Our results suggest that chronic exposure to lead pollution from mining activities may cause disturbances in the chemical composition of both mineral and organic composition of bone tissue inferred by FTIR. In addition, levels of Pb in bone were positively related with the crystallinity index obtained by X-ray powder diffraction, indicating a possible direct effect of Pb exposure on bones strength. These results suggest that mining pollution may affect bone mineralization processes in exposed wild animals. doi:10.1016/j.toxlet.2010.03.445 P108-045 Acute effects of pyrene on the common goby pomatoschistus microps (Teleostei, Gobiidae) M. Oliveira 1 , L.R. Vieira 1 , C. Gravato 1 , L. Guilhermino 2 1 CIIMAR - Centro Interdisciplinar de Investigac ¸ ão Marinha e Ambiental, Universidade do Porto, Portugal, 2 CIIMAR - Centro Interdisciplinar de Investigac ¸ ão Marinha e Ambiental, Universidade do Porto, Laboratório de Ecotoxicologia; ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Departamento de Estudos de Populac ¸ ões, Laboratório, Portugal Coastal marine areas are exposed to a multitude of anthropogenic stressors that include polycyclic aromatic hydrocarbons (PAHs). Pyrene is the dominant PAH produced from incomplete combus- tion of oil and oil products being present in the environment due to oil spills and seeps, among other processes. Thus, the assessment of its impact on the biota is of undeniable importance, especially due to the limited number of studies that have been conducted dealing with its effects on fish. In this perspective, the current study focused on the acute effects of pyrene on the common goby Pomatoschis- tus microps, a fish species with a relevant role in trophic webs. Juveniles were individually exposed for 96 h to a concentration