Mycol. Res. 99 (2): 23G232 (1995) Printed in Great Britain 230 Three new species of Pseudocercospora from the Nepal Himalaya USHA BUDATHOKI1 AND SANJAY K. SINGHZ Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal Department of Botany, University of Gorakhpur, U.P. India Pseudocercospora heterospermi sp. nov. on Heferospermurn pedunculosum Linn., P. himalayana sp. nov. on Triumfetta pilosa Roth. and P. smilacis sp. nov. on Smilax aspera Linn., collected from Kathmandu Valley in Nepal Himalaya, are described, illustrated and compared with allied taxa. Pseudocercospora heterospermi Budathoki & S. K. Singh sp. nov. (Fig. I) Maculae hypogenae, nervisequae, atrae, extendentes per 1/3 vel toturn folii; mycelium ex hyphis imrnersis, hyalinis, ramosis, laevibus; stromata substomatoidea, 16-26 x 13-26 pm. Conidiophora rnacro- nernatosa, mononematosa, caespitosa, recta vel flexuosa, plerumque ramosa, septata, simplicia, laevia, pallide olivacea vel bmnnea, Fig. 1. Pseudocercospora heterospermi, showing stroma (A), conidio- phores (B), branched conidiophore (C) and conidia (D). Bar 20 prn. 32.5-65.5 x 2.54.5 pm. Cellulae conidiogenae in conidiophorsis incorporatae, denticulatae, polyblasticae. Conidia solitaria, simplicia, laevia, 3-7 septata, brunnea, 29-58.5 x 34.5 pm. In foliis vivis Heterospermi pedunculosi (Asteraceae), Godawari, Kathmandu Valley, Nepal, Nov. 1986, leg. Usha Budathoki, KU 60 holotypus, IMI 291865 isotypus. Colonies strictly hypogenous, vein limited, blackish patches coalescing and covering almost one third or more of the leaf surface. Mycelium composed of immersed, hyaline, branched, smooth-walled hyphae. Stromafa substomatal, bearing fas- ciculate conidiophores, more or less globose, pseudo- parenchymatous, 16-26 x 13-26 pm. Conidiophores macro- nematous, mononematous, caespitose, straight or flexuous, sometimes branched, septate, simple, smooth, pale-olivaceous brown, fasciculate, 32.5-65.5 x 2-5-6.5 ym. Conidiogenous cells integrated, slightly denticulate, terminal, polyblastic. Conida solitary, simple, smooth, transversely 3-7 septate, brown, measuring 29-58.5 x 3-6-5 ym. There are no previous records of a Pseudocercospora species described on the host genus. However, 20 species of Pseudocercospora have so far been described on the Asteraceae (Deighton, 1976, 1987; Yen & Lim, 1980, 1983; Shukla ef al., 1982; Khan & Shamsi, 1983; Goh and Hsieh, 1989; Sarbajana & Das, 1990; Braun & Castaiieda, 1991). Amongst these 20 species, the well developed stromata and branching in the conidiophores of the present species are shared only by Pseudocercospora chysanfhmicola (J. M. Yen) Deighton and P. cinereae (Pavgi & U. P. Singh) Deighton. The present species is, therefore, compared with these and differences are shown in Table 1. It is clear that P. heterospermi is closer to P. cinereae in having a similar number of septa in the conidia but their dimensions are significantly different. As regards the length of conidio- sphores, it is closer to P. chysanthemicola but differs in the length and septation of the conidia. Branched conidiophores