Vol. 14, 1995 Notes 451 6. Farhoud-Moghaddam AA, Katouli M, Jafad A, Bahavar MA, Parst M, Matek Zadeh F: Antimicrobial drug re- sistance and resistance factor transfer among clinical isolates of salmonellae in Iran. Scandinavian Journal of Infectious Diseases 1990, 22: 197-203. 9. Shehabi AA, Zubi J, Za'balawi G: Emergence of multiple drug resistance among Salmonella species in Jordan. European Journal of Clinical Microbiology 1983, 4: 351- 354. 10. Bauer AW, Kirby WM, Sherris JC, Turck M: Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 1966, 45: 493-496, 11. National Committee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7- A2. NCCLS, Villanova, PA, 1990. 12. Kado CL, Liu ST: Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacte- riology 1981, 145: 1365-1373. 13. Ackerman VP, Obbink D J: Screening for transferable antibiotic resistance in the clinical laboratory. Joumal of Antimicrobial Chemotherapy 1979, 5: 167-172. 14. Roberts F J: Nontyphoidal, nonparatyphoidal salmonella septicemia in adults. European Journal of Clinical Mi- crobiology & Infectious Diseases 1993, 12: 205-208. 15. Green SDR, Cheesbrough JS: Salmonella bacteremia among young children at a rural hospital in Western Zaire. Annals of Tropical Paediatrics 1993, 13: 45-54. 16. Threlfall E, Hall M, Rowe B: Salmonella bacteremia in England and Wales, 198t-1990. Journal of Clinical Pathology 1992, 45: 34-36. 17. Cohen JI, Bartlett JA, Corey GR: Extraintestinatmanife- stations of salmonella infections. Medicine 1987, 4: 349- 387. 18. Shehabi AA, Haddadin MM: Salmonella serotypes iso- lated from patients at two main medical centers in Amman, 1976-1980. Jordan Medical Journal 1982, 16: 46-54. 19. Khuri-Bulos NA, Abu Khalaf M, Shehabi A, Shami K: Foodhandler-associated salmonella outbreak in a uni- versity hospital despite routine surveillance cultures of kitchen employees. Infection Control and Hospital Epi- demiology 1994, 15: 311-314. 20. Wittier RR, Bass JW: Nontyphoidal salmonella enteric infections and bacteremia. Pediatrics Infectious Disease Journal 1989, 8: 364-367. 21. Shehabi AA: Epidemiology of antimicrobial resistance among Shigella isolates over five years. Jordan Medical Journal 1984, 18: 19-26. 22. Gulig PA: Virulence plasmids of Salmonella typhimurium and other salmonellae. Microbial Pathogenesis 1990, 8: 3-11. 23. Helmuth R, Stephan R, Bunge C, Hoog B, Steinbeck A, Bulling E: Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serotypes. Infection and Im- munity 1985, 48: 175-182. 24. Fierer J, Krause M, Taxue R, Guiney D: Salmonella typhimufium bacteremia: association with the virulence plasmid. Journal of Infectious Diseases 1992, 166: 639- 642. Isolation of a Bordetella avium-Like Organism from a Human Specimen C. Dorittke 1, P. Vandamme 2, K.H. Hinz 3, E.M. Schemken-Birk 1, C.H. Wirsing yon K6nig 1. The isolation of a strain of Bordetella for which the species could not be determined but which most closely resembled Bordetetla avium is reported. The strain was isolated in mixed culture from an ear swab of a patient suffering from chronic otitis media. The bacterium showed the typical biochemi- cal reactions of Bordetella avium but differed in an- timicrobial resistance pattern, protein and fatty acid composition, and DNA-DNAand DNA-rFINA hybrid- ization. Further studies will clarify the taxonomic sta- tus of this strain within the Bordetella-Alcaligenes ribosomal RNA cluster. Apart from Bordetella pertussis and Bordetella parapertussis, for which human ciliated epithelial cells are the only habitat, the genus Bordetella in- cludes other species such as Bordetella bronchi- septica and Bordetella avium, which are rarely if ever associated with human disease (1). In partic- ular, Bordetella avium and 'Bordetella avium-like organisms' have been found only in poultry (2). We report the isolation of an unusual member of the genus Bordetella which closely resembles Bor- detella avium but does not completely fit into any of the known species of the genus Bordetella. Materials and Methods. The ear swab from which the bacteria were isolated was taken from a 29-year-old male prior to tympanotomy. The swab was transported in Amies medium and processed the same day in the microbiological laboratory. Swabs were streaked onto sheep blood agar, chocolate agar and MacConkey agar and immersed in a broth containing liver extracts 1Institute for Hygiene and Laboratory Medicine, St~idtische Krankenanstalten Krefeld, Lutherplatz 40, D-47805 Krefeld, Germany. 2Department of Microbiology, University of Ghent, Belgium. 3Klinik fiat Gefl~gelkrankheiten, Tier~irztliche Hochschule Hannover, Germany.