ORIGINAL PAPER Saturnispora serradocipensis sp. nov. and Saturnispora gosingensis sp. nov., two ascomycetous yeasts from ephemeral habitats Monica R. Canelhas • Anne C. Barbosa • Adriana O. Medeiros • Ching-Fu Lee • Li-Yin Huang • Marc-Andre ´ Lachance • Carlos A. Rosa Received: 26 April 2010 / Accepted: 25 June 2010 / Published online: 6 July 2010 Ó Springer Science+Business Media B.V. 2010 Abstract Two novel ascomycetous yeast species, Saturnispora serradocipensis and Saturnispora gosing- ensis, were isolated from leaf detritus in a tropical stream of Southeastern Brazil and a mushroom collected in Taiwan, respectively. Analysis of the D1/D2 domains of the large-subunit of the rRNA gene of these strains showed that these species are related to Saturnispora hagleri, their closest relative. Saturnispora serradocip- ensis and S. gosingensis differed from S. hagleri, respectively, by seven nucleotide substitutions and two indels and three nucleotide substitutions and three indels in D1/D2 rRNA sequences. The two new species differ from each another by four nucleotide substitutions and one indel in D1/D2 rRNA sequences. However, the ITS sequences of S. serradocipensis, S. gosingensis and S. hagleri were quite divergent, showing that they are genetically separate species. The type strain of S. serradocipensis is UFMG-DC-198 T (=CBS 11756 T = NRRL Y-48717 T ), and of S. gosingensis GA4M05 T is (CBS 11755 T = NRRL Y-48718 T ). Keywords New yeast species Á Saturnispora serradocipensis Á Saturnispora gosingensis Á Leaf detritus Á Mushroom Introduction The Saturnispora clade (Saccharomycetales) contains seven recognized species, S. ahearnii, S. besseyi, S. dispora, S. hagleri, S. mendocae, S. saitoi, and S. zaruensis. Six anamorphic Candida species, C. diversa, C. sanitii, C. sekii, C. siamensis, C. silvae and C. suwanaritii, are also related to this clade (Morais et al. 2005; Kurtzman 2006; Boonmak et al. 2009; Limtong et al. 2010). Most species of Satur- nispora were isolated from plant-related substrates, soil, and water samples. These yeasts are character- ized by the fermentation and assimilation of only a few carbon compounds and are almost indistinguish- able physiologically from one another (Kurtzman 2006; Boonmak et al. 2009; Limtong et al. 2010). During a survey of yeasts associated with leaf detritus in a tropical stream of Southeastern Brazil, M. R. Canelhas Á A. C. Barbosa Á C. A. Rosa (&) Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil e-mail: carlrosa@icb.ufmg.br A. O. Medeiros Departamento de Bota ˆnica, IB, Universidade Federal da Bahia, Salvador, BA 40170-115, Brazil C.-F. Lee Á L.-Y. Huang Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu 300, Taiwan, ROC M.-A. Lachance Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada 123 Antonie van Leeuwenhoek (2011) 99:241–247 DOI 10.1007/s10482-010-9482-9