MEETING ABSTRACTS Open Access 2nd WAO International Scientific Conference (WISC 2012), Abstracts Hyderabad, India. 6-9 December 2012 Edited by Ruby Pawanker, Lanny J Rosenwasser and Stephen T Holgate Published: 23 April 2013 These abstracts are available online at http://www.waojournal.org/supplements/6/S1 MEETING ABSTRACTS P1 Asthma diagnosis and treatment – 1001. Identification of prevalent sensitizing allergens in India Shubnum Singh Allied Health & Wellness Programs, New Delhi, India World Allergy Organization Journal 2013, 6(Suppl 1):P1 Background: This study was conducted to identify the most common sensitizing food and inhalant allergens in physician-diagnosed allergic children and adults in North India. Methods: 274 allergy-diagnosed patients, divided into Group A (aged 6– 12 yrs) and Group B (aged 12–65 yrs), were enrolled in the study. They were classified as atopic if had at least one positivity when screened with ImmunoCAP® Phadiatop and fx5 (Food mix 5; 6 common foods), a technology considered as the gold standard for IgE antibody blood testing worldwide. For identification of the sensitizing allergens atopic patients were further tested by ImmunoCAP® Specific IgE using a broad panel of common Indian allergens covering 17 foods and 19 inhalants (singles/ mixes). Total IgE level was also determined for each atopic patient. Results: Phadiatop/fx5 determined 59% (162/274) of the patients as atopic, where of 159 were included in further evaluation; 10% were in Group A and 90% were in Group B. Higher proportion (36%) of patients had the medical history of urticaria followed by atopic dermatitis (26%), asthma (23%) and rhinitis (23%). The commonest sensitizing food allergen was banana (68%) followed by sesame seeds (66%), lemon (45%), rice (31%), wheat (24%), cashew (23%) and peanut (21%). Among inhalants, house dust mite, D. farinae (83%) was the most prevalent sensitizing allergen followed by cockroach (79%), weed pollens (29-50%), tree pollen (16-29%), grass pollen mix (26%) and mold mix (25%). Less than 20% of patients tested positive to cow’s milk, tomato, spinach, aubergine, soybean, egg white, dog dander, legume mix, chicken, cat dander and fish. The geometric mean of total IgE was 943 kU/l (21->5000 kU/l, range 2-5000 kU/l). Conclusions: This is the first Indian sensitization data of this dignity analyzed by ImmunoCAP® which provided useful native information of prevalent sensitizing Indian allergens that would improve cost effectiveness of allergy treatment and hence increase the quality of life of allergic patients in India. Phadiatop/fx5 revealed that the physicians’ diagnosis of IgE mediated allergy was accurate only in 59% of cases and thus highlights the importance of using allergy tests in conjunction with clinical findings for correct allergy diagnosis. P2 Asthma diagnosis and treatment – 1002. FEF25-75%: a more sensitive indicator in the early detection of asthma Marzieh Tavakol 1* , Mohammad Gharagozlou 2 , Mohsen Afaride 3 , Masoud Movahedi 3 , Zahra Tavakol 3 1 Allergy and Clinical Immunology, Tehran University Of Medical Sciences, Children Medical Center, Tehran, Iran; 2 ISAA, Cipla, India; 3 Tehran University of Medical Sciences, Children Medical Center, Tehran, Iran World Allergy Organization Journal 2013, 6(Suppl 1):P2 Background: Spirometry is widely regarded as a clinically invaluable measurement method that is of genuine recommend for the diagnosis and management of asthmatic patients. FEV-1 and FEV1/FVC are vastly perceived as asthma severity and control assessment indices, according to the present clinical guidelines. Since FEV-1 index is chiefly within normal ranges even in the most severe cases, the certain criteria for asthma diagnosis is immensely base upon it’s significant alteration after bronchodilator challenge test. FEF25-75% represents a well-established indicator of small airway disease now for decades, however, and It has been demonstrated that asthmatic patients do have remarkably lower FEF25-75%. Additionally FEF 25-75% meaningful response to the bronchodilator challenge test is seen in some asthmatics that are healthy in terms of other prognostic parameters. Objective: This study was designed to detect the most sensitive index for the diagnosis of asthma and to determine the correlation between FEV-1 and FEF25-75% indices and asthma control questionnaire (ACQ) scores. Methods: we recruited 107 patients with the diagnosis of asthma who were attending follow-up sessions at the Children’ s Medical Center Hospital (CMCH) between December 1, 2010 and May 31, 2012 to conduct a hospital- based study. A p value if <0.05 was considered to be clinically significant to our study. Results: FEF25-75% Response proved to be more sensitive in detection of asthma in comparison to FEV-1 Response as shown in our study (p = 0.042). Nevertheless, Pre-bronchodilator FEF 25-75% does not follow this trend, compared to Pre-bronchodilator FEV-1 (p = 0.69). FEF 25-75% Response and ACQ score were significantly correlated (p = 0.01) while this is not the case between FEV-1 Response and ACQ score (p = 0.46). Moreover, Pre- bronchodilator FEF 25-75 had a meaningful relationship with ACQ score (p = 0.03), as opposed to the pre-bronchodilator FEV-1 in which no significant correlation was seemingly spotted (p = 0.17). World Allergy Organization Journal 2013, Volume 6 Suppl 1 http://www.waojournal.org/supplements/6/S1 © 2013 various authors, licensee BioMed Central Ltd. All articles published in this supplement are distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.