NEW GENOMIC TOOLS FOR MOLECULAR STUDIES OF EVOLUTIONARY CHANGE IN THREESPINE STICKLEBACKS by DAVID M. KINGSLEY 1,2,3) , BAOLI ZHU 4) , KAZUTOYO OSOEGAWA 4) , PIETER J. DE JONG 4) , JACQUELINE SCHEIN 5) , MARCO MARRA 5) , CATHERINE PEICHEL 6) , CHRIS AMEMIYA 7) , DOLPH SCHLUTER 8) , SARITA BALABHADRA 2) , BRIAN FRIEDLANDER 2) , YEE MAN CHA 9) , MARK DICKSON 9) , JANE GRIMWOOD 9) , JEREMY SCHMUTZ 9) , WILLIAM S. TALBOT 2) and RICHARD MYERS 9,10) ( 1 HHMI; 2 Dept. of Developmental Biology, Stanford, CA USA 94305-5329; 4 BACPAC Resources, CHORI, Oakland, CA USA; 5 Genome Sciences Center, BC Cancer Agency, Vancouver BC V5Z4E6 Canada; 6 Fred Hutchinson Cancer Research Center, Seattle, WA USA; 7 Molecular Genetics Department, Benaroya Research Institute at Virginia Mason, Seattle WA; 8 Dept. of Zoology, University of British Columbia, Vancouver BC V6T1Z4; 9 Dept. of Genetics and Stanford Human Genome Center, Stanford, CA USA) (Acc. 30-IX-2004) Summary The dramatic radiation of sticklebacks in different post-glacial environments provides a unique opportunity to study the molecular mechanisms that underlie rapid evolutionary change in vertebrates. We have developed a number of genomic and genetic tools to facil- itate further study of a wide range of morphological, physiological and behavioral traits in sticklebacks. A large collection of microsatellite markers has previously been developed for use in genome-wide linkage mapping of interesting traits in crosses between different stick- leback forms. cDNA libraries have been generated and EST sequencing projects have begun to isolate stickleback homologs of developmental control genes. Large insert BAC libraries have been built to compare chromosome regions of interest from both anadromous and fresh- water stickleback populations. Large scale fingerprinting of one of these libraries has been 3) Corresponding author; e-mail address: kingsley@cmgm.stanford.edu 10) We gratefully acknowledge the assistance of Qing Cao and Teresa Ren with respect to BAC insert size determination. This work was supported by National Institutes of Health grant HG002568 from the Center of Excellence in Genomic Science program (DMK, RMM, WST). Work at CHORI was also supported in part by NIH grant 5U01HG002523-01 (PJdJ). DMK is an associate investigator of the Howard Hughes Medical Institute. © Koninklijke Brill NV, Leiden, 2004 Behaviour 141, 1331-1344 Also available online -