Downloaded from www.microbiologyresearch.org by IP: 54.147.156.167 On: Fri, 03 Jun 2016 01:41:42 Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea Ying-Shun Cui, 1,2 Wan-Taek Im, 1 Cheng-Ri Yin, 2 Jung-Sook Lee, 3 Keun Chul Lee 3 and Sung-Taik Lee 1 Correspondence Wan-Taek Im wandra@kaist.ac.kr Sung-Taik Lee e_stlee@kaist.ac.kr 1 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea 2 Key Laboratory of Natural Resources of the Changbai Mountain and Functional Molecules (Yanbian University), Ministry of Education, Yanji 133002, China 3 Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong-gu, Daejeon 305-333, Republic of Korea A Gram-positive, rod-shaped, non-spore-forming and strictly aerobic bacterium (Gsoil 161 T ) was isolated from soil of a ginseng field in Pocheon Province, South Korea. The novel isolate was characterized using a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 161 T was shown to belong to the family Nocardioidaceae and was related to Aeromicrobium marinum (98.0 % similarity to the type strain), Aeromicrobium alkaliterrae (97.6 %), Aeromicrobium fastidiosum (97.0 %) and Aeromicrobium erythreum (96.7 %); the sequence similarity with other species within the family was less than 94.4 %. It was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in the cell- wall peptidoglycan, MK-9(H 4 ) as the predominant menaquinone and C 16 : 0 , 10-methyl C 18 : 0 (tuberculostearic acid), C 16 : 0 2-OH, 10-methyl C 17 : 0 and 10-methyl-C 16 : 0 as the major fatty acids. The G+C content of the genomic DNA was 65.5 mol%. These chemotaxonomic properties and phenotypic characteristics support the affiliation of strain Gsoil 161 T to the genus Aeromicrobium. Results of physiological and biochemical tests enabled strain Gsoil 161 T to be differentiated genotypically and phenotypically from currently known Aeromicrobium species. Therefore, strain Gsoil 161 T represents a novel species, for which the name Aeromicrobium panaciterrae sp. nov. is proposed. The type strain is strain Gsoil 161 T (=KCTC 19131 T =DSM 17939 T =CCUG 52476 T ). The genus Aeromicrobium was proposed by Miller et al. (1991). According to the original description, the genus comprised non-mycelial, non-sporulating actinomycetes that produced the macrolide antibiotic erythromycin; the type species is Aeromicrobium erythreum. In 1994, Nocardioides fastidiosa was transferred to the genus as Aeromicrobium fastidiosum (Tamura & Yokota, 1994). Two further species, Aeromicrobium marinum (Bruns et al., 2003) and Aeromicrobium alkaliterrae (Yoon et al., 2005), were described recently. In this study, taxonomic characterization is reported of an Aeromicrobium-like strain, Gsoil 161 T , which was isolated from soil of a ginseng field in South Korea during a study on the culturable aerobic bacterial community. Strain Gsoil 161 T was originally isolated from soil of a ginseng field in Pocheon Province, South Korea. This soil sample was thoroughly suspended in 50 mM phosphate buffer (pH 7.0) and the suspension was spread on one- tenth-strength modified R2A (l 21 : 0.25 g tryptone, 0.25 g peptone, 0.25 g yeast extract, 0.125 g malt extract, 0.125 g beef extract, 0.25 g Casamino acid, 0.25 g soytone, 0.5 g glucose, 0.3 g soluble starch, 0.2 g xylan, 0.3 g sodium pyruvate, 0.3 g K 2 HPO 4 , 0.05 g MgSO 4 , 0.05 g CaCl 2 , 15 g agar) agar plates after serial dilution with 50 mM phosphate buffer (pH 7.0). Plates were incubated at 30 u C for 1 month. Single colonies on the plates were purified by transferring them onto new plates and these were incubated again on modified R2A or half-strength modified R2A. Purified colonies were tentatively identified by partial 16S rRNA gene sequences (Im et al., 2005). Strain Gsoil 161 T was one of the isolates that appeared on the modified R2A agar plates under aerobic conditions. As it could grow well on full- strength modified R2A agar and commercial R2A agar Abbreviation: DAP, diaminopimelic acid. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Gsoil 161 T is AB245387. 64697 G 2007 IUMS Printed in Great Britain 687 International Journal of Systematic and Evolutionary Microbiology (2007), 57, 687–691 DOI 10.1099/ijs.0.64697-0