Animal Feed Science and Technology 123–124 (2005) 313–327 Defaunating capacity of tropical fodder trees: Effects of polyethylene glycol and its relationship to in vitro gas production Gregorio E. Monforte-Brice˜ no, Carlos A. Sandoval-Castro ∗ , Luis Ram´ ırez-Avil´ es, Concepci ´ on M. Capetillo Leal Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut´ onoma de Yucat´ an, Apdo. 4-116 Itzimn ´ a, M´ erida, Yucat´ an 97100, M´ exico Abstract The defaunating effect of multipurpose fodder trees, and the effect of polyethylene glycol (PEG) on this capacity, were evaluated with Acacia angustissima, A. gaumeri, A. farnesiana, Bursera simaruba, Calliandra calothyrsus, Enterolobium cyclocarpum, Erythrina indica, E. standleyana, Gliricidia mac- ulata, G. sepium, Guazuma ulmifolia, Leucaena leucocephala, Lysiloma latisiliquum, Piscidia pis- cipula and Vitex gaumeri. The crude protein (CP) content ranged from 112, in V. gaumeri, to 266 g/kg dry matter (DM) in L. leucocephala. The fodder trees with highest CP content were L. leucocephala (266 g/kg DM), E. indica (213), G. maculata (216) and G. sepium (208). The neutral detergent fibre content ranged from 283 to 515 g/kg DM and ADF from 166 to 402 g/kg DM. In E. cyclocarpum and A. angustissima polyphenol, condensed tannins (CT) and saponins were detected. In vitro gas production and protozoa numbers were negatively correlated with CT content in the absence of PEG and, in both the presence (P = 0.057) and absence (P = 0.001) of PEG, with saponins. Saponins had a strong correlation with gas production with (P = 0.074) and without (P = 0.089) PEG. L. latisiliquum, B. simaruba, A. angustissima, C. calothyrsus and A. farnesiana had CT contents higher than 100 g/kg DM. Addition of PEG increased gas production in A. angustissima, A. farnesiana, C. calothysus and L. latysiliquum, but had an effect on IVDMD and IVOMD in only A. angustissima and A. farnesiana. Protozoa counts decreased to zero by 24 h in A. farnesiana, C. calothyrsus, L. latisiliquum and E. Abbreviations: CP, crude protein; CT, condensed tannins; DM, dry matter; NDF, neutral detergent fibre; PEG, polyethylene glycol; TP, total phenols; IVDMD, in vitro apparent; DM, digestibility; IVOMD, in vitro apparent; OM, digestibility; IVGP, in vitro gas production; OM, organic matter; SCFA, short chain fatty acids ∗ Corresponding author. Tel.: +52 999 942 32 00; fax: +52 999 942 32 05. E-mail address: ccastro@tunku.uady.mx (C.A. Sandoval-Castro). 0377-8401/$ – see front matter © 2005 Elsevier B.V. All rights reserved. doi:10.1016/j.anifeedsci.2005.04.016