Int.J.Curr.Microbiol.App.Sci (2016) 5(4): 793-802 793 Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.504.091 Implementation on diet of Probiotic Saccharomyces spp.SB-6 isolated from colon of Bali cattle on egg production and egg cholesterol concentration of Lohmann brown laying hens I Gusti Nyoman Gde Bidura 1* , Ida Bagus Gaga Partama 1 , Dewa Ketut Harya Putra 1 and Urip Santoso 2 1 Faculty of Animal Husbandry, Udayana University, Denpasar-Bali, Indonesia 2 Department of Animal Science, Faculty of Agriculture, Bengkulu University, Indonesia *Corresponding author ABSTRACT Introduction It is widely recognized that, in most developing countries, people need to increase their animal protein consumption. Thus, attempt must be made to enhance livestock production, particularly those that have rapid growth rate and short generation time such as poultry. Concerning poultry production, while efforts have been made to improve the production, the low level of people’s consumption of animal protein is further constrained by the ignorance and unwilling of people to eat eggs due to its high cholesterol content that is believed may lead to health problem. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 4 (2016) pp. 793-802 Journal homepage: http://www.ijcmas.com The current study was aimed to study the effect of addition on diet of various levels of probiotic Saccharomyces spp.SB-6, isolated from colon of Bali cattle, on egg productions and its total cholesterol levels of Lohmann Brown layers. A total number of 120 layers at 32 weeks of age and with homogenous body weight were randomly divided into 4 treatment groups. Each treatment group consisted of 6 replications and with 5 birds in each of it. Therefore, 24 battery colony pens were provided. The four treatment groups were assigned based on their diets, namely treatments B, C, and D in which their diets were supplemented with 0.20, 0.40, and 0.60 g of probiotic/kg of diet, respectively, whereas there was no probiotic supplementation for treatment A which was considered as the control group. All experimental birds were fed ad libitum. Six eggs from each treatment group were collected randomly for assessment of cholesterol content following standard procedure. The present results showed that, compared to control birds, layers that subjected to probiotic supplementation have a significant increase (P<0.05) in egg production (hen-day production) and in the total egg weight, but showed a slight decrease in serum and egg cholesterol contents. Thus, it may be concluded that addition on diet of probiotic Saccharomyces spp.SB-6 at various level has positive effects on chicken production and, therefore, it can be recommended widely to chicken farmers. Keywords Probiotic, Cholesterol Level, Hen-Day Production, Lohmann Brown Layers. Accepted: 24 March 2016 Available Online: 10 April 2016 Article Info