Neuroinformatics © Copyright 2006 by Humana Press Inc. All rights of any nature whatsoever are reserved. 1539-2791/06/65–80/$30.00 (Online) 1559-0089 DOI: 10.1385/NI:4:1:65 Original Article Abstract The thorough characterization of transgenic mouse models of human central nervous sys- tem diseases is a necessary step in realizing the full benefit of using animal models to investi- gate disease processes and potential therapeutics. Because of the labor- and resource-intensive nature of high-resolution imaging, detailed investigation of possible structural or bio- chemical alterations in brain sections has typi- cally focused on specific regions of interest as determined by the researcher a priori. For example, Parkinson’s disease researchers often focus imaging on regions of the brain expected to exhibit pathology such as the substantia nigra and striatum. Because of limitations in acquiring and storing high-resolution imaging data, additional data contained in the specimen is not usually acquired or disseminated/reported to the research community. Here we present a method of imaging large regions of brain at close to the resolution limit of light microscopy using a mosaic imaging technique in conjunc- tion with multiphoton microscopy. These maps are being used to characterize several geneti- cally modified animal models of neurological disease by filling the information “gap” among techniques such as magnetic resonance imaging and electron microscopic analysis. Index Entries: Large-scale imaging; animal models; multiphoton; database; Biomedical Informatics Research Network. (Neuroinformatics DOI: 10.1385/NI:4:1:65) High-Resolution Large-Scale Mosaic Imaging Using Multiphoton Microscopy to Characterize Transgenic Mouse Models of Human Neurological Disorders Diana L. Price, Sunny K. Chow, Natalie A. B. MacLean, Hiroyuki Hakozaki, Steve Peltier, Maryann E. Martone, * and Mark H. Ellisman National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, Department of Neurosciences, University of California, San Diego, CA 92093-0608 65 *Author to whom all correspondence and reprint requests should be addressed. E-mail: mmartone@ucsd.edu