1 The mycobacterial gene cuvA/Rv1422 is required for optimal nutrient utilization and 1 virulence 2 Mushtaq Mir 1*+ , Sladjana Prisic 1+ , Choong-Min Kang 1** , Shichun Lun 2 , Haidan Guo 2 , Jeffrey P. 3 Murry 3*** , Eric J. Rubin 3 , Robert N. Husson 1# 4 5 1 Division of Infectious Diseases, Children’s Hospital, Harvard Medical School, Boston, MA, 6 02115 7 2 Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, 8 MD, 21231 9 3 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, 10 MA 02115 11 *Current address: Division of Genetics, Wadsworth Center, Center for Medical Science, New 12 York State Department of Health, Albany, NY 12201 13 **Current address: Department of Biological Sciences, California State University Stanislaus, 14 Turlock, CA 95382 15 ***Current address: Gilead Sciences, 333 Lakeside Drive, Foster City, CA 94404 16 + These authors contributed equally to this work. 17 Running title: Mycobacterial cuvA: nutrient utilization and virulence 18 Key words: Mycobacterium, cholesterol, bacterial cell pole, peptidoglycan synthesis, virulence, 19 cell wall, Rv1422 20 #Corresponding author information: 21 Robert N. Husson 22 Division of Infectious Diseases 23 Children’s Hospital Boston 24 300 Longwood Ave. 25 Boston, MA 02115 26 Tel 617-919-2900/Fax 617-730-0254 27 robert.husson@childrens.harvard.edu 28 IAI Accepts, published online ahead of print on 21 July 2014 Infect. Immun. doi:10.1128/IAI.02207-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved. on June 13, 2016 by guest http://iai.asm.org/ Downloaded from