1 Copyright © 2008 by ASME Proceedings of the Sixth International ASME Conference on Nanochannels, Microchannels and Minichannels ICNMM2008 June 23-25, 2008, Darmstadt, Germany ICNMM2008-62294 TIME OPTIMIZATION OF HETEROGENEOUS IMMUNOASSAY USING PDMS MICROFLUIDIC CHIP FOR QUANTITATIVE DETECTION OF A MODEL ANALYTE Peng Li 1 , Assem Abolmaaty 2 , Thomas Barek 1 , Constantine Anagnostopoulos 1 , Mohammad Faghri 1, * 1 Department of Mechanical Engineering and Applied Mechanics, University of Rhode Island, Kingston, RI, 02881, U.S.A. 2 Food Science Department, Faculty of Agriculture, Ain Shams University, Cairo, 11241, Egypt ABSTRACT * Corresponding author. Tel.: +1-401-874-5180; fax: +1-401-874-2355. Email address: lip@egr.uri.edu (P. Li), faghrim@egr.uri.edu (M. Faghri). Heterogeneous immunoassay (HI) has been recently used for on-chip biomolecule detection by many researchers due to its high specificity and precision. However, the protocols that have been published are too time-consuming. For HI based immunosensor to be attractive they should have short analysis times. In our work, a polydimethylsiloxane (PDMS) microfluidic device was fabricated to quantify a model analyte (bovine IgG) and used for optimizing the time required for each process. The results show that the incubation time of one single process could be reduced from typical 1 hour to 5 minutes without loss of performance. Our studies reveal that microfluidic devices are only effective in reducing incubation times for diffusion limited processes but not for interaction or adsorption limited process. 1 INTRODUCTION In the past decade, polydimethylsiloxane (PDMS) microfluidic chips have been utilized extensively in various research fields. One of the burgeoning areas is on-chip immunoassay with attractive and important applications in point-of-care diagnostics and in-the-field analysis [1]. Recent advances in immunotechnology made the detection and identification of biological analytes more convenient, more sensitive, and more specific than conventional assays [2]. The basic principle of immunoassay is the binding of antibodies to a target antigen, followed by the detection of the antigen-antibody complex. Biologists take advantage of the fact that antibodies have characteristics that make them very useful for a variety of medical and biological studies, such as the detection of food borne pathogens. In particular, antibodies typically are exquisitely specific for a particular antigen (especially well selected monoclonal antibodies) and have extremely high binding affinities for antigens. This makes them quite valuable for detecting particular antigens. Nowadays many diagnostic tests are based on immunological techniques. Of the various immunoassay formats that could possibly be used in microfluidic devices, the heterogeneous immunoassay (HI) is the predominant analytical technique for quantitative determination of a broad variety of analytes in clinical diagnosis, plant pathology, the food industry, and environment monitoring. Despite its high specificity and precision, HI is a time consuming, multi-step process that requires long incubation periods. It often takes many hours to as much as two days to perform one assay. The detection time consumed is very critical in saving people’s lives and reducing substantial economic cost on the society. Therefore, for HI based microfluidic immunosensor to be attractive they should have short analysis times. Several microfluidic chips have been developed based on HI procedures. Sato and coworkers [3] integrated an immunosorbent assay into a glass microchip for the detection of human secretory immunoglobulin A (s-IgA). They were able to reduce the time necessary for the antigen-antibody reaction from 24 h to less than 1 h. Hayes and coworkers [4] developed a HI with packed paramagnetic particles within a microchannel. The time required for direct interaction of fluorescein isothiocyanate (FITC) with immobilized anti-FITC was only 3 min. Lai and coworkers [5] reported a compact disk-like microfluidic platform that performed HI for rat IgG from a hybridoma cell culture. The enzymatic reaction took only 200 seconds (120 min in a microtiter plate). The reason for these dramatic reductions in processing time is because the diffusion time required for the molecule to adsorb onto the solid surface is proportional to the square of the characteristic length of the channel [6]. In the published literature, only the specific antigen-antibody reaction time has been optimized among all HI steps. However, the time required for other steps such as coating, blocking, and washing should also be optimized as the