Berumen et al. – Chaetodon vagabundus microsatellites Page 1 / 8 Isolation and characterization of 15 polymorphic nuclear microsatellite primers for 1 the widespread Indo-Pacific vagabond butterflyfish, Chaetodon vagabundus 2 3 Michael L. Berumen* 1,2 , Elisabeth Rochel 3 , Glenn R. Almany 2 , Simon R. Thorrold 1 , Geoffrey 4 P. Jones 2,4 , Morgan S. Pratchett 2 , Craig Syms 4 , and Serge Planes 3 5 6 1. Biology Department, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, 7 02543, USA. 8 9 2. ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, 10 Queensland, 4811, Australia. 11 12 3. UMR 5244, CNRS-EPHE-UPVD, Centre de Biologie et d’Ecologie Tropicale et 13 Méditerranéenne, Université de Perpignan, 66860 Perpignan Cedex, France. 14 15 4. School of Marine and Tropical Biology, James Cook University, Townsville, Queensland, 16 4811, Australia. 17 * corresponding author: 18 Michael Berumen 19 M.S. #50 20 266 Woods Hole Road 21 Woods Hole, MA, 02543, USA 22 mberumen@whoi.edu 23 24 Keywords: cross-amplification, Chaetodon vagabundus, Chaetodon baronessa, microsatellites, 25 speciation 26 Running title : Chaetodon vagabundus microsatellites 27