Eur J Forest Res (2009) 128:117–128 DOI 10.1007/s10342-008-0221-y 123 ORIGINAL PAPER Nutrient contents and eYciencies of beech and spruce saplings as inXuenced by competition and O 3 /CO 2 regime Hermann Rodenkirchen · Axel Göttlein · Alessandra R. Kozovits · Rainer Matyssek · Thorsten E. E. Grams Received: 13 March 2007 / Revised: 20 March 2008 / Accepted: 26 May 2008 / Published online: 19 June 2008  Springer-Verlag 2008 Abstract Saplings of Fagus sylvatica and Picea abies were grown under conditions of intra and interspeciWc com- petition in a 2-year phytotron study under combinations of ambient and elevated ozone (+O 3 which is 2 £ O 3 , but <150 nl l ¡1 ) as well as carbon dioxide concentrations (+CO 2 which is amb. CO 2 + 300 l CO 2 l ¡1 ) in a full facto- rial design. Saplings were analysed for various mineral nutrients in diVerent plant organs as well as biomass pro- duction and crown development. The study was based on the assumption that nutritional parameters important for growth and competitiveness are aVected by stress defence under limiting nutrient supply. The hypotheses tested were (1) that nutrient uptake-related parameters (a) as well as eYciencies in nutrient use for above-ground competition (b) of beech rather than spruce are impaired by the expo- sure to elevated O 3 concentrations, (2) that the eYciency in nutrient uptake of spruce is enhanced by elevated CO 2 concentrations in mixed culture, and (3) that the ability to occupy above-ground space at low nutrient cost is co-deter- minant for the competitive success in mixed culture. Clear nitrogen deWciencies were indicated for both species during the 2-year phytotron study, although foliar nitrogen-biomass relationships were not so close for spruce than for beech. O 3 stress did not impair nutrient uptake-related parameters of beech; thus hypothesis (1a). was not supported. A negative eVect of elevated O 3 (under amb. CO 2 ) on the N and P based eYciencies in above-ground space occupation (i.e. lower crown volume per unit of N or P invested in stems, limbs and foliage) of beech supported hypothesis (1b). It appeared that ozone stress triggered a nutrient demand for stress defence and tolerance at the expense of above-ground com- petition (trade-oV). Crown volume of beech under O 3 stress was stabilized in monoculture by increased nutrient uptake. In general, the +CO 2 -treatment was able to counteract the impacts of 2 £ O 3 . Elevated CO 2 caused lower N and S concentrations in current-year foliage of both tree species, slightly higher macronutrient amounts in the root biomass of spruce, but did not increase the eYciencies in nutrient uptake of spruce in mixed culture. Therefore hypothesis (2) was not supported. At the end of the experiment spruce turned out to be the stronger competitor in mixed culture as displayed by its higher total shoot biomass and crown volume. The amounts of macronutrients in the above-ground biomass of spruce individuals in mixed culture distinctly exceeded those of beech, which had been strongly reduced by inter- speciWc competition. The superior competitiveness of spruce was related to higher N and P-based eYciencies in above- ground space occupation as suggested in hypothesis (3). Communicated by A. RoloV. This article belongs to the special issue “Growth and defence of Norway spruce and European beech in pure and mixed stands”. H. Rodenkirchen (&) · A. Göttlein Forest Nutrition and Water Resources, Department of Ecology, Technische Universität München, Am Hochanger 13, 85354 Freising, Germany e-mail: dr.hermann.rodenkirchen@t-online.de A. Göttlein e-mail: goettlein@forst.tu-muenchen.de A. R. Kozovits Departamento de Ecologia, Universidade de Brasilia, Caixa Postal 04457, Brasilia, DF 70919-970, Brazil R. Matyssek · T. E. E. Grams Ecophysiology of Plants, Department of Ecology, Technische Universität München, Am Hochanger 13, 85354 Freising, Germany