71 Revista Ibérica de Aracnología, nº 22 (30/06/2013): 71–78. ARTÍCULO Grupo Ibérico de Aracnología (S.E.A.). ISSN: 1576 - 9518. http://www.sea-entomologia.org/ SECOND ANNOTATED LIST OF SELECTED SPIDER FAMILIES (ARACHNIDA: ARANEAE) HELD AT THE UNIVERSITY OF LEÓN (SPAIN)* J. Benhadi-Marín 1,3 , J. A. Barrientos 3 , M. A.Ferrández 3 , S. Henriques 4 & E. Morano 5 1 Mountain Research Centre (CIMO), School of Agriculture, Polytechnic Institute of Bragança, Bragança, Portugal – jbenma@hotmail.com 2 Unidad de Zoología, Facultad de Biociencias, Universidad Autónoma de Barcelona, Barcelona, Spain. 3 Sociedad para el Estudio y Conservación de las Arañas (SECA) C/Villafranca, 24 (1º) 28028, Madrid, Spain. 4 Terrestrial Invertebrates Division, Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD. 5 Santo Tomás de Villanueva, 79-A. 13170 - Miguelturra (Ciudad Real), Spain. Abstract: The diversity and distribution of spiders in the Iberian Peninsula are poorly known. However, the institutional arthropod collections that remain unexplored can provide a considerable amount of taxonomical and biogeographical data. Here we analyze the samples of the families Agelenidae, Dysderidae, Eresidae, Lycosidae, Nemesiidae and Pholcidae stored at the University of León (Spain). The 30 species that have been identified are here commented upon, and the male and female genitalia of Holocnemus caudatus, Holocnemus hispanicus and Holocnemus pluchei are presented. Also, a predictive distribution map of Ne- mesia ungoliant is provided. The review of the CaULE collection has proved to be a very efficient way to access previously un- tapped biodiversity information. Key words: Collection, taxonomy, biogeography, Agelenidae, Dysderidae, Eresidae, Lycosidae, Nemesiidae, Pholcidae, Iberian Peninsula. Segunda lista comentada de varias familias de arañas (Arachnida: Araneae) depositadas en la Universidad de León (España) Resumen: El conocimiento sobre la diversidad y distribución de las arañas Ibéricas está lejos de ser completo. Sin embargo, una gran cantidad de colecciones de artrópodos mantenidas en las diferentes instituciones que permanecen inexploradas en la actualidad pueden ser una valiosa fuente de datos taxonómicos y biogeográficos. En este trabajo analizamos las muestras de las familias Agelenidae, Dysderidae, Eresidae, Lycosidae, Nemesiidae y Pholcidae, conservadas en la Universidad de León (España). En total se identificaron 30 especies. Se aportan comentarios faunísticos y el dibujo de la estructura genital de ambos sexos de Holocnemus caudatus, Holocnemus hispanicus y Holocnemus pluchei. Además se proporciona un mapa de distribución potencial de Nemesia ungoliant. La revisión de la colección CaULE ha demostrado ser un medio muy eficaz para acceder a información de biodiversidad sin explotar. Palabras clave: Colección, taxonomía, biogeografía, Agelenidae, Dysderidae, Eresidae, Lycosidae, Nemesiidae, Pholcidae, península Ibérica. * Nota editorial: En este volumen se publica otro manuscrito relativo a la aracnofauna de León y Asturias: Arácnidos (Arachnida: Araneae y Opiliones) recogidos durante las XI Jornadas del GIA en Somiedo (Asturias), septiembre 2010. Marcos Méndez Igle- sias, Eduardo Morano y Carlos E. Prieto: pág. 131–135. Introduction Studies on the Iberian spider fauna have considerably in- creased in the last decade, with crucial data being compiled on the known species and their distribution (Morano & Car- doso, 2011). This ground work has allowed easier access to the previously published information, but large data sets re- main unavailable to the scientific community, held in uniden- tified material in museums and university collections. Even in well studied groups such as plants, it is predict- ted that 50% of undescribed species have already been co- llected and are just waiting in herbaria to be analyzed: there- fore, it becomes clear that research resources should be fo- cused as much on examining museum material as on collec- ting new material in the field (Bebber et al., 2010). As the collecting effort has clearly been not as intense with spiders as it is with plants, we cannot accurately assess the informative value of museum collection for arachnids, but Spain provides a good example of a territory in which a con- siderable amount of taxonomical and biogeographical data has been obtained from institution holdings (Barrientos, 1978; Barrientos, 1979; Barrientos et al., 1983; Barrientos & Ferrández, 1982; Barrientos & Urones, 1985; Hidalgo, 1983; Morano & Benhadi-Marín, 2012). In a time of increasingly limited funding resources, to review collections scattered in decentralised institutions has proven to be a very efficient way to access previously un- tapped biodiversity information (e.g. Morano & Benhadi- Marín, 2012). This data may be particularly valuable in poor- ly studied groups distributed across biodiversity hotspots, such as the case study of Iberian spiders, where the number of new species described has consistently increased in the last years. In addition, the asymptote still seems far to reach, a high level of endemicity of 236 species has been recorded and a fifth of the known species are only recorded from a single locality, with 50% of the species records coming from five localities or fewer (Morano & Cardoso, 2011). The aim of the present paper is to contribute to the ta- xonomical and biogeographical knowledge of the Spanish spider fauna by analyzing previously undetermined samples from the families Agelenidae, Dysderidae, Eresidae, Lyco- sidae, Nemesiidae and Pholcidae, held at the University of