Abstracts / Journal of Biotechnology 136S (2008) S356–S401 S383 Pandya, P.H., Jasra, R.V., Newalkar, B.L., Bhatt, P.N., 2005. Studies on the activity and stability of immobilized -amylasein in ordered mesoporous silicas. Micropor. Mesopor. Mater. 77, 67–77. Wan, Y.Y., Du, Y.M., Shi, X.W., 2006. Immobilization and characterization of lac- case from Chinese Rhus vernicifera on modified chitosan. Process Biochem. 41, 1378–1382. doi:10.1016/j.jbiotec.2008.07.880 V3-P-074 Comparison of 4-nitro-1,8-naphthalic anhydride biotransfor- mation using bakers’ yeast and its crude homogenates detected by RP-HPLC Jianqiang Xu 1 , Qing Yang 1,∗ , Xuhong Qian 2 , Jörgen Samuelsson 3 , Jan-Christer Janson 3,∗ 1 Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China 2 Shanghai Key Laboratory of Fine Biology, East China University of Science and Technology, Shanghai 200237, China 3 Department of Surface Biotechnology, Uppsala Biomedical Center, Uppsala University, P.O. Box 577, Uppsala SE-751 23, Sweden E-mail address: qingyang@dlut.edu.cn (Q. Yang). Microorganisms which are able to reduce a wide range of nitroarene compounds are known and have become a valuable asset in biodegradation and biotransformation processes. Recent investiga- tions demonstrated bakers’ yeast exhibited high chemoselectivity in the reduction of nitroarene compounds to their correspond- ing arylhydroxylamines or amines (Li et al., 2004). In this study, a simultaneous detection of reduction products by RP-HPLC was used (Chen et al., 2005) and the efficiency difference in 4-nitro-1,8- naphthalic anhydride (4-NNA) biotransformation was compared between bakers’ yeast and its crude homogenates. The detec- tion limit of 4-hydroxylamino-1,8-naphthalic anhydride (Li et al., 2005) and 4-amino-1,8-naphthalic anhydride are 20 ng and 10 ng, respectively, when 10 l of sample was injected. The whole-cell bakers’ yeast exhibited higher chemoselectivity than the crude homogenates in the reduction of 4-NNA into its correspond- ing hydroxylamine, 4-hydroxylamino-1,8-naphthalic anhydride (4-HANA). However, crude homogenates were more efficient in the reduction of 4-NNA into 4-amino-1,8-naphthalic anhydride (4- ANA). Possible reason is that the whole cell provides sustainable reducing force, which promotes and stabilizes the arylhydrox- ylamines biotransformation. Since both nitroarene amines and arylhydroxylamines are important starting materials for some drugs’ synthesis, the data obtained here might guide choices of biocatalysts in the form of whole cells or homogenates. Acknowledgements Financial support by the Swedish government through its agency VINNOVA is gratefully acknowledged. This study was also under the auspices of 973 Project (2003CB114400), 863 Project (2003AA2Z3520), the Fok Ying Tung Education Founda- tion (101072) and the National Natural Science Foundation of China (20536010, 20576016 and 20676021). References Chen, G.S., Lambert, I.B., Douglas, G.R., White, P.A., 2005. Assessment of 3- nitrobenzanthrone reductase activity in mammalian tissues by normal-phase HPLC with fluorescence detection. J. Chromatogr. B 824, 229–237. Li, F., Cui, J.N., Qian, X.H., Zhang, R., 2004. A novel strategy for the preparation of aryl- hydroxylamines: chemoselective reduction of aromatic nitro compounds using bakers’ yeast. Chem. Commun., 2338–2339. Li, F., Cui, J.N., Qian, X.H., Zhang, R., Xiao, Y., 2005. Highly chemoselective reduction of aromatic nitro compounds to the corresponding hydroxylamines catalysed by plant cells from a grape (Vitis vinifera L.). Chem. Commun., 1901–1903. doi:10.1016/j.jbiotec.2008.07.881 V3-P-075 Effect of surfactant on asymmetric bioreduction of 2-octanone catalyzed by whole cell of Saccharomyces cerevisiae Shi Xian’ai 1,2,∗ , Rong Jinlei 1 , Li Yongning 2 , Guo Yanghao 2 , Zong Minhua 1 1 Laboratory of Applied Biocatalysis, South China University of Tech- nology, Guangzhou 510640, China 2 College of Biological Sciences & Technology, Fuzhou University, Fuzhou 350002, China E-mail address: shixa@fzu.edu.cn (S. Xian’ai). Enantiomeric pure 2-octanol is an important chiral chemical for preparation of ferroelectric and antiferroelectric liquid crystals with intrinsic second-order nonlinear optical activity. Furthermore, it is also used as a building block for synthesis of many chiral drugs. In this paper, the effects of surfactants on this reaction catalyzed by Saccharomyces cerevisiae were investigated systematically (Laouar et al., 1996; Aguedo et al., 2004; Goswami et al., 2000). The results showed that cationic, anionic and nonionic surfactants in the bio- transformation system could not only decrease the reverse and side reaction rate, but also increase the desired reaction rate. The fur- ther investigation suggested that the content and some properties of surfactants, such as the kind of charge in hydrophobic group, molecular weight and HLB (Hydrophile–Lipophile-Balance) value, were the major factors which influence the reaction. Comparing with cationic and anionic surfactants, nonionic surfactant is the best one in improving the yield and enantiomeric excess (e.e.) of (S)- 2-octanol in the reaction. The presence of the nonionic surfactants, Tween-20, improved the yield and e.e. markedly after 96 h reac- tion, at a content 0.4 mmol L -1 of surfactant. Under this condition, the yield and e.e. value would be 93.16% and 99.31%, which were higher than that in the control, 63.72% in yield and 62.44% in e.e. value, respectively. It was indicated that surfactants play an impor- tant role on asymmetric bioreduction of 2-octanone with whole cell of Saccharomyces cerevisiae. The presence of nonionic surfac- tant at a proper content will boost the reaction and enhance the enantioselectivity. References Aguedo, M., Wache, Y., Coste, F., Husson, F., Belin, J.M., 2004. Impact of surfac- tants on the biotransformation of methyl ricinoleate into gamma-decalactone by Yarrowia lipolytica. J. Mol. Catal. B: Enzym. 29 (1–6), 31–36. Goswami, A., Bezbaruah, R.L., Goswami, J., Borthakur, N., Dey, D., Hazarika, A.K., 2000. Microbial reduction of omega-bromoacetophenones in the presence of surfactants. Tetrahedron: Asymmetry 11 (18), 3701–3709. Laouar, L., Lowe, K.C., Mulligan, B.J., 1996. Yeast responses to nonionic surfactants. Enzyme Microb. Technol. 18 (6), 433–438. doi:10.1016/j.jbiotec.2008.07.882