23eau AN EXPERIMENTAL STUDY IN NUDE MICE ON THE ELECTROMAGNETIC DIAGNOSIS OF TUMORS Andrea Tubaro, Antonella Stoppacciaro°, Cosimo De Nunzio, Carlo Leonetti*, Gabriella Zupi* and Lucio Miano Departments of Urology and Pathology° , Sant’Andrea Hospital, “La Sapienza” University, Rome, Italy *Laboratory for Preclinical Experimental Chemotheraphy, National Cancer Institute “Regina Elena”, Rome, Italy  Electromagnetic detection of prostate cancer with the TRIMprob is a new promising technology. Preliminary data on the diagnostic accuracy of the TRIMprob at 465 MHz intensity, suggested a higher sensitivity and a slightly lower specificity compared PSA, free/total PSA ratio with better positive and negative predictive values (1-3). No experimental data are avilable. Aim of our study was to:  To measure signal intensity at 465, 930 and 1395 MHz in a experimental prostate and breast tumours  To define the lowest tumour size identified by the TRIMprob  To explore the possible relation between tumour size and signal intensity  To investigate the effect of the site of tumour implantation on signal intensity OBJECTIVES MATERIALS AND METHODS ABS: 830 REFERENCES 1) Tubaro A, De Nunzio C, Trucchi A et al.: , The ehe elettromagnetic detection of prostatic cancer: evaluation of diagnostic accuracy. Urology 2008 in press. 2) Bellerofonte C, Vedrucio C, Tombolino P et al. Non-Invasive Detection of Prostate Cancer by Electromagnetic Interaction. Eur. Urol. 47,29-37, 2005 3) Da Pozzo L, Scattoni V, Mazzoccoli B et al.: Tissue-resonance interaction method for the noninvasive diagnosis of prostate cancer: analysis of a multicentre clinical evaluation. BJU INT, 100, 1005-9, 2007 SCANNING CONDITIONS AND TECHNIQUE  A significantly reduction of signal intensity at 465 MHz was observed in nude mice with prostate or breast cancer after tumour growth in the prostate cancer model.  A significant correlation was found between tumour weight and  Change of signal intensity (465 MHz) from baseline  Signal intensity (465 MHz) at 3 weeks post-implant  Data from our experimental tumour models confirm the diagnostic value of the TRIMprob for the electromagnetic diagnosis of cancerous tissue  Confirmation studies are currently ongoing in prostate and breast cancer CONCLUSIONS RESULTS I: Baseline scanning: male vs female RESULTS III: Subcutaneous tumour implant; Signal intensity at 3 weeks  30 nude mice (15 ♂ and 15 ♀)  Week -1  Animals received and stabilised in our animal facility for a week  Marked for further identification  Week 0  All animals examined at baseline with the TRIMprob  Week 1 to 3  5 + 5 mice injected every week, for 3 weeks with PC3 (prostate) or CG5 (breast) cells  10 5 cells suspended in HBSS (50 microL)  Injection site: left tight (intramuscular), right shoulder (subcutaneous)  Week 4  TRIMprob analysis performed in injected mice  Tumour size was measured in all animals  Week 5  Animals were then sacrificed, all tumours were excised, fixed and processed for histology.  Room tested for EM pollution  Anaesthetised animals  2m distance between animal/probe and receiver  All tests done in triplicate  Single investigator Sex N Mean* SD SE 465 MHz Male 15 151.6 12.8 3.31 Female 15 157.0 15.1 3.90 Independent-Sample T test, p≤ n.s. 154 95 128 64 137 117 0 20 40 60 80 100 120 140 160 180 200 465 MHz 930 MHz 1395 MHz Baseline 3 weeks RESULTS II: Intramuscular tumor implant signal intensity at 3 weeks 153 103 119 98 123 116 0 20 40 60 80 100 120 140 160 180 200 465 MHz 930 MHz 1395 MHz Baseline 3 weeks P≤ 0.0001 P≤ 0.0001 P≤ 0.05 P≤ 0.0001 P≤ 0.01 P≤ 0.58 465 MHz 3 weeks Δ 465MHz Baseline-3 weeks Tumor Weight σ : - 0.682 p=0.005 σ : 0.63 p= 0.01 RESULTS V: All mice, correlation of signal intensity at 465 MHz and tumour weight PC3 CG5 i.m. s.c. i.m. s.c. Mean (mg) 914 211 906 276 Range 320-3323 62-799 387-1568 67-784 RESULTS IV: Tumor weight Independent-Sample T test, p≤ n.s. 830--P A. Tubaro Novel therapies for prostate cancer