RJC RASĀYAN JOURNAL OF CHEMISTRY Vol.1,No.1(2008),55-58 A VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LETROZOLE IN BULK AND SOLID DOSAGE FORM M.Ganesh* 1 , K.Kamalakannan 1 , Rahul Patil 1 , Satish Upadhyay 1 , Anand Srivatsava 1 , T.Sivakumar 1 and Swastika Ganguly 2 1 Department of Pharmaceutical Analysis, Nandha College of Pharmacy, Koorapalayam Pirivu,Erode – 638 052. Tamilnadu, India. 2 Department of Pharmaceutical Sciences, Birla Inst. Of Tech., Mesra, Ranchi. Johrkhand, India E.Mail:chemgans@gmail.com ABSTRACT A simple, sensitive, sectrophotometric method in UV region has been developed for the determination of Letrozole is bulk and tablet dosage form. Standard solution of Letrozole shows maximum absorbance at240nm with apparent molar absorptive of 3.3016 × 10 4 l/mol/cm. Beers law was obeyed in the concentration range of 1 -10 μg /m.l with regression ,slope and intercept 0.9998,-0.016,0.1164 respectively. Result of the analysis was validated statically and by recovery studies. The results percentage recovery is100.63±0.4215 which shows that the method is free form interference of additives and impurities during the estimation drug in formulation. This shows the adoptability of the method for the routine quality control analysis of the drug in bulk and in formulation. Keywords: Letrozole, UV determination, bulk, table formulation. INTRODUCTION Letrozole is 4, 4’ – (1H-1, 2, 4-triazole-1yl methylene) dibenzonitrile (Fig-1). It is an anti-cancer agent, which is used for the treatment for the breast cancer caused by the hormonal imbalance 1,2 . It is a nonsteroidal competitive inhibitor of the aromatase enzyme system (adrenal androgens) there by inhibiting the biosynthesis of estrogen. A few HPLC method have been reported for Letrozole in biological fluids 3-8 .There is no UV method of estimation reported for Letrozole.The present study is aimed to develop and selective, precise accurate and reliable UV method for determination of Letrozole in bulk and solid dosage forms. EXPERIMENTAL Instruments and reagents: An Elico UV –Visible spectrophotometer SL-164, with 1cm matched quartz cells was used for the absorbance measurements, All the chemicals used were of analytical grade. Pure letrozole obtained as a gift sample from Sanmar chemicals Chennai, two commercial samples of the drug tablets were procured from the market. Standard Preparation: 100mg of pure drug was transferred to a 100ml standard volumetric flask. Small amount of absolute ethanol was added to dissolve the drug. Then the volume was made up to the mark with absolute ethanol to get stock solution. UV SPECTROPHOTOMETRIC METHOD M.Ganesh et al.