Asian Journal of Chemistry Vol. 21, No. 9 (2009), 7431-7433 NOTE RP-HPLC Estimation of Desloratadine in Pharmaceutical Dosage Form J. VALARMATHY*, L. SAMUEL JOSHUA†, N.T.H. GUPTHA, M. GANESH, A. LAKSHMANA RAO‡ and T. SIVA KUMAR Nandha College of Pharmacy, Koorapalayam Pirivu, Erode-638 052, India E-mail: valarmathyjoshua@rediffmail.com A simple, rapid, sensitive and precise HPLC method has been developed for the estimation of desloratadine in pharmaceutical dosage form. In this method RP-C18 column (250 mm × 4.6 mm i.d., 5 μm particle size) with mobile phase consisting of acetonitrile, 0.05 M phos- phate buffer and methanol in the ratio of 48:45:7 v/v/v in isocratic mode was used. The detection wavelength is 247 nm and the flow rate is 0.8 mL/min. In the range of 20-100 μg/mL, the linearity of desloratadine shows a correlation coefficient of 0.998. The percentage recovery ranges from 99.98-100.30 %. The proposed method was validated by deter- mining sensitivity, accuracy, precision and linearity. Key Words: Desloratadine, HPLC, Validation. Desloratadine is an orally administered nonsedative, long acting antihistaminic with selective H 1 -receptor antagonistic activity. Desloratadine is an active metabolite of loratadine. Chemically 1 desloratadine is 8-chloro-6,11-dihydro-11-(4-piperi- dinylidene)-5H-benzo[5,6]cyclohepta[1,2-b]pyridine. Literature survey reveals that various HPLC methods 2-10 have been reported for the estimation of desloratadine in pharmaceutical dosage forms. The present method is simple, rapid, sensitive, accurate and precise HPLC method for the determination of desloratadine in bulk as well as in tablet dosage form. The separation was carried out on isocratic HPLC system (Shimadzu) with Shimadzu Binary HPLC pump, Shimadzu LC-10AT UV-Visible Detector, Spinchrom software and RP-C 18 column (250 mm × 4.6 mm i.d.; particle size 5 μm). HPLC conditions: The mobile phase consisting of acetonitrile (HPLC grade), 0.05 M phosphate (KH 2 PO 4 ) buffer (pH adjusted to 3.0 with orthophosphoric acid) of AR grade and methanol (HPLC grade) were filtered through 0.45 μ membrane filter before use, degassed and were pumped from the solvent reservoir in the ratio †Department of Pharmaceutical Chemistry, Padmavathi College of Pharmacy and Research Institute, Krishnagiri Main Road, Periyanahalli, Dharmapuri-635 205, India. ‡Shri Vishnu College of Pharmacy, Bhimavaram-534 202, India.