Original Research Article International Journal of Pharmaceutical Chemistry and Analysis, April-June, 2016;3(2):73-79 73 Development and Validation of Stability Indicating HPTLC Method for Estimation of Rizatriptan Benzoate in Bulk and Tablet Dosage Form Pritam S. Jain 1,* , Harshal P. Chaudhari 2 , Sanjay J. Surana 3 1 Associate Professor, 2 PG Student, 3 Principal, R.C. Patel Institute of Pharmaceutical Education & Research, Maharashtra *Corresponding Author: Email: pritash79@yahoo.com Abstract A new simple, accurate, precise and selective stability-indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for the determination of Rizatiptan Benzoate in bulk and tablet formulation. Chromatographic separation was performed on aluminum plate precoated with Silica Gel 60 F254 using, Methanol: n-Propane: Triethylamine (3:5:2 v/v/v) as mobile phase followed by densitometric scanning at 280 nm. This system was found to give compact spot for Rizatiptan Benzoate (Rf value of Rf = 0.38 ± 0.02). The calibration curve was found to be linear between 800- 2800 ng/band. The limit of detection and quantitation were found to be 2.24 ng/band and 6.79 ng/band respectively. The proposed method can be applicable for the estimation of Rizatiptan Benzoate during stability studies. Keywords: Rizatiptan Benzoate, High Performance Thin Layer Chromatography (HPTLC), Stability-Indicating Method. Access this article online Quick Response Code: Website: www.innovativepublication.com DOI: 10.5958/2394-2797.2016.00011.3 Introduction Rizatriptan benzoate is synthetically portrayed as: N, N-dimethyl-5-(1H-1,2,4-triazol-1-ylmethyl)- 1H- indole-3-ethanamine (Fig. 1) [1] . It is a particular 5- hydroxyl triptamine 1B/1D receptor agonist to remember headache cerebral pains. Its experimental equation is C15H19N5 and its atomic weight is 269.34486 g/mol. Flow hypotheses on the etiology of migraine propose that indications are because of nearby cranial vasodilatation and/or to the arrival of vasoactive and professional provocative peptides from tactile nerve finishing in an enacted trigeminal framework. [2-4] Since there are just couple of strategies are accessible for the determination of Rizatriptan benzoate. The present work is an endeavor to evaluate the same by a New HPTLC technique [5-7] . The writing audit demonstrates not very many techniques for the determination of Rizatriptan benzoate and pharmaceutical approvals by HPTLC strategy yet that different strategies like UV spectroscopic technique for Rizatriptan benzoate, HPLC technique for Rizatriptan benzoate, LC-MS and LC-MS/MS strategy for determination of Rizatriptan benzoate in human plasma [8] . This strategy can be effectively utilized for routine examination of Rizatriptan benzoate as it is fast, basic, specific and touchy strategy for the determination utilizing High Performance Thin Layer Chromatographic (HPTLC) system [9] . Materials and Methods Instruments: The HPTLC system (Camag Switzerland) consisting of Linomat V semiautomatic spotting device, TLC Scanner IV (Camag Muttenz, Switzerland), twin-trough developing chamber (10 x 10cm), UV cabinet with dual wavelength UV lamps, Win-CATS software, syringe (100 ȝl capacity, Hamilton) were used for chromatographic study. Electronic analytical balance (Shimadzu AUX-220) was used for all the weighing. Reagents and Materials: Rizatriptan Benzoate was received as a gift sample from Cipla Ltd., Mumbai. Methanol, chloroform, ammonia solution were of analytical grade (MERCK Chem. Ltd., Mumbai). Rizatriptan Benzoate formulation was purchased from local market. Chromatographic Conditions: Chromatographic separation was performed on 10 × 10 cm aluminum backed plates pre-coated with 250 ȝm layer of silica gel 60 F254 (E. Merck, Darmstadt, Germany). The TLC plates were prewashed with methanol and dried in oven at 50⁰C for 30 min. Samples were spotted on TLC plate 15 mm from the bottom edge by Linomat V semi- automatic spotter using following parameters: band width, 6 mm; track distance, 11.6 mm; application rate, 0.1ȝl/s. The TLC plate was developed in twin trough chamber using Methanol: n-Propanol: Triethylamine (3:5:2 v/v/v) as mobile phase at temperature, 25±2˚C; chamber saturation time, 15 min; migration distance, 75 mm. The TLC plate was scanned and analyzed by TLC Scanner IV and WinCATS software using following