ISSN 2320-5407 International Journal of Advanced Research (2016), Volume 4, Issue 6, 2110-2117 2110 Journal homepage: http://www.journalijar.com INTERNATIONAL JOURNAL Journal DOI: 10.21474/IJAR01 OF ADVANCED RESEARCH RESEARCH ARTICLE COMPARATIVE STUDIES OF L-LACTICACID PRODUCTION FROM GROUND NUT SHELL AND SUGARCANE MOLASSES BY MUTANT LACTOBACILLUS DELBRUECKIINCIM2025 U-25 STRAIN. C.Obula Reddy 1 , AVNSwamy 1 , D. Muralidhara Rao 2 . 1. Department of Biotechnology, ChaitanyaBharathi Institute of Technology, Gandipet Hyderabad, Telanaga,India. 2. Department of Biotechnology, Srikrishna Devaraya University, Anantapuramu, Andhra Pradesh, India. Manuscript Info Abstract Manuscript History: Received: 14 April 2016 Final Accepted: 19 May 2016 Published Online: June 2016 Key words: Ground nut shell , mutation , lactic acid , UV radiation. *Corresponding Author C.Obula Reddy. The lactic acid production from groundnut shells is economical since the raw material is cheaply available source even though the yield of lactic acid is about 30g/lit. This would be a potential source for producing the lactic acid. However the lactic acid from other sources like sugarcane molasses is tried to enhance the yield. It is observed that lactic acid yield is 23.5g/lit from the sugar concentration of 20g/lit.Which is more than the strains without mutations, which is about 20g/lit. Mutants were generated by exposing to UV radiations, with different exposure times(5,10,15,20 and 25 min) and stability is tested on 0.6%Allyl aicohol.Among the mutants the results fromUV-25 strain were encouraging and the lactic acid produced was about 30 g/l from groundnut shells and 23.5g/lit from sugarcane molasses at temperature of 42 0 c&40 0 C. The pH optimum was found to be about5.5 & 5.2 and inoculum size was found to be 5ml& 3 ml. The present comparative studies were focused on the concentration of lactic acid from groundnut shell and sugarcane molasses by mutant strain U-25. Copy Right, IJAR, 2016,. All rights reserved. Introduction:- Lactic acid is a monomer which is used to produce a biopolymer PLA (Polylactic Acid), and PLA can be a good alternative to the polymer produced by petrochemical route(Rojan et.al.,2009). Lactic acid demand is expected to increase due to the development of new, large-volume uses, particularly as a feedstock for biodegradable polylactic acid (PLA) polymers, oxygenated chemicals (Datta and Henry, 2006).Most of the lacto bacilli and Lactococci were found to ferment xylose(Colliansand James ,1984) . The yield coefficient of lactic acid was reported by (Tanaka and komiyama2002) , and found that it exceeded 1mol/mol in cultivations when initial xylose concentrations were more than 50 g/l. However the fermentative rate of xylose by pentose –fermentative bacteria is lower than that of glucose by homo fermentative lactic acid bacteria such as L.casei,(Garde et.al(2002) . A mathematical model to simulate simultaneous saccharification and lactic acid fermentation was proposed by( Luo et.al 1997). It is very expensive when sugars, e.g., glucose, sucrose, starch, etc., are used as the feedstock for lactic acid production. Therefore, lignocellulosic biomass is a promising feedstock for lactic acid production considering its great availability, sustainability, and low cost compared to refined sugars. Despite these advantages, the commercial use of lignocellulose for lactic acid production is still problematic. This review describes the ―conventional‖ processes for producing lactic acid from lignocellulosic materials with lactic acid bacteria. These processes include: pretreatment of the biomass, enzyme hydrolysis to obtain fermentable sugars, fermentation technologies, and separation and purification of lactic acid.(Mohammad Ali et.al 2011). Both starchy and lignocellulosic biomass have been extensively used, however lignocellulosic biomass in Ghana is generated in large volumes as crop residues and mostly considered waste although some amount is used as animal feed. These crop residues are readily available as cheap raw materials for lactic acid production. By employing appropriate fermentation processes about 199,856