Synthetic peptide issued from Hap1/LipL32 for new early serodiagnosis of human leptospirosis F. Aviat a,c,1 , S. Rochereau-Roulet a , C. Branger b,2 , J.M. Estavoyer c,3 , B. Chatrenet d,4 , J.L. Orsonneau e,5 , C. Thorin a , G. Andre-Fontaine a, * a Unite ´ de Bacte ´riologie Me ´dicale et Mole ´culaire des Leptospires, Ecole Nationale Ve ´te ´rinaire de Nantes, BP 40 706, 44 307 Nantes Cedex 03, France b Centre for Infectious Diseases and Vaccinology, Biodesign Institute, Arizona State University, AZ, USA c Service des Maladies Infectieuses et Tropicales, Centre Hospitalier Universitaire Saint Jacques, 25000 Besanc ¸on, France d Virbac, 13e `me rue LID, BP 27, 06 511 Carros Cedex, France e Institut de Biologie Humaine, Centre Hospitalier Universitaire Ho ˆtel Dieu, 44 000 Nantes, France Accepted 31 January 2009 Abstract Leptospirosis is a worldwide zoonosis. Today, serological diagnosis is generally assessed by MAT. We performed ELISA with a synthetic peptide derived from Hap1/lipL32 which is a protein expressed only by pathogenic Leptospira. Repeatability and thresholds were defined with 85 controls sera and 119 hospitalized leptospirosis. The PP-ELISA repeatability and IgM/IgG cut-off values were based on control sera. For these cut-off values, we observed the IgM-PP-ELISA specificity of 89%, whereas it was 100% for the IgG. www.elsevier.com/locate/cimid Available online at www.sciencedirect.com Comparative Immunology, Microbiology and Infectious Diseases 33 (2010) 375–387 * Corresponding author. Tel.: +33 2 40 68 76 91; fax: +33 2 40 68 76 94. E-mail addresses: florence_aviat@yahoo.fr (F. Aviat), rochereau@vet-nantes.fr (S. Rochereau-Roulet), branger_christine@yahoo.fr (C. Branger), jmestavoyer@chu-besancon.fr (J.M. Estavoyer), bchatrenet@virbac.fr (B. Chatrenet), jeanluc.orsonneau@chu-nantes.fr (J.L. Orsonneau), thorin@vet-nantes.fr (C. Thorin), andre-fontaine@vet-nantes.fr (G. Andre-Fontaine). 1 Present address: Unité de Biologie des Spirochètes, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France. Tel.: + 33 1 45 68 83 66/33 2 40 68 77 77. 2 Tel.: +480 727 8322. 3 Tel.: +33 3 81 66 81 66. 4 Tel.: +33 4 92 08 71 00. 5 Tel.: +33 2 40 08 33 33. 0147-9571/$ – see front matter # 2009 Elsevier Ltd. All rights reserved. doi:10.1016/j.cimid.2009.01.001