for 50 implants. It is shown that the dose for critical organ varied dramatically for each different implant. Such detailed dose in- formation is not available for traditional film-based planning. Our experience also showed that significant shrinkage of both gross tumor and normal uterus occurred with successive brachytherapy fractions resulting in a lower point A dose (Figure 2). Conclusions: 3D HDR allows for dose volume analysis of both CTV and organs at risk. While treatment outcome evaluation is the final objective of our study, we have evaluated and reported volumetric dose coverage of CTV and organs at risks. Longer follow- up is still necessary to determine optimal dose delivery to target tissues while sparing normal tissues. Author Disclosure: B. Wang, None; Y. Zhu, None; I. Yeo, None; A.K. Kwon, None; C. Henson, None. 2342 Comparison of Glucose Transporter-1 Expression With Cellular Hypoxia in Normal Epithelium, CIN and Squamous Cell Carcinoma of the Uterine Cervix M. A. Varia 1 , S. Chou 1 , Y. Azuma 1 , R. A. Lininger 1 , L. Van Le 1 , D. E. Thrall 2 , J. A. Raleigh 1 1 University of North Carolina, Chapel Hill, NC, 2 North Carolina State University, Raleigh, NC Purpose/Objective(s): This investigation examines possible reasons for the weak relationship between glucose transporter-1 (Glut-1) expression and hypoxia in squamous cell carcinoma (SCC) of the uterine cervix. Materials/Methods: Patients with SCC of the uterine cervix enrolled in an Institutional Review Board approved tumor hypoxia study protocol were infused with the hypoxia marker pimonidazole hydrochloride. Cervical tumor biopsies were obtained in 33 patients the day following the pimonidazole infusion. Biopsies were formalin-fixed, paraffin-embedded, sectioned and immuno- stained for Glut-1, Factor VIII, PCNA, metallothionein I/II (associated with proliferating cells), involucrin (associated with cells at intermediate and end stages of keratinocyte differentiation) and pimonidazole adducts. Biopsies containing normal epithelium and cervix intraepithelial neoplasia (CIN) were sectioned and immunostained for Ki-67 (MIB-1), metallothionein I/II, CK19, HIF-1a, Glut-1, involucrin and pimonidazole adducts. Results: Glut-1 was expressed in oxic basal cells in normal epithelium. Glut-1 expression expanded greatly in CIN but in a way unrelated to hypoxia. Glut-1 expression appeared in both oxic and hypoxic cells in SCC. No correlation between Glut-1 expression and hypoxia (r 2 = 0.02; p = 0.43), microvascular density (r 2 = 0.02; p = 0.48) or tumor grade was observed. A statistically significant S394 I. J. Radiation Oncology d Biology d Physics Volume 69, Number 3, Supplement, 2007