Solid-State and Solution Structural Studies of 4-{[C(E)]-1H-Azol-1- ylimino)methyl}pyridin-3-ols by Dionisia Sanz* a ), Almudena Perona a ), Rosa M. Claramunt a ), Elena Pinilla* b ), M. Rosario Torres b ), and José Elguero c ) a ) Departamento de Química Orgánica y Bio-Orgánica, Facultad de Ciencias, UNED, Senda del Rey9, ES-28040 Madrid (fax: + 34 913988372; e-mail: dsanz@ccia.uned.es) b ) Departamento de Química Inorgánica, Laboratorio de Difracción de Rayos X, Facultad de Ciencias Químicas, Universidad Complutense, ES-28040 Madrid (e-mail: epinilla@quim.ucm.es) c ) Instituto de Química Médica (CSIC), Centro de Química Orgánica ‘Manuel Lora Tamayo’, Juan de la Cierva 3, ES-28006 Madrid The new N-salicylideneheteroarenamines 1 4 were prepared by reacting the biologically relevant 3- hydroxy-4-pyridinecarboxaldehyde (5) with 1H-imidazol-1-amine (6), 1H-pyrazol-1-amine (7), 1H-1,2,4- triazol-1-amine (8), and 1H-1,3,4-triazol-1-amine (9). Solution 1 H-, 13 C-, and 15 N-NMR were used to establish that the hydroxyimino form A is the predominant tautomer. A combination of 13 C- and 15 N- CPMAS-NMR with X-ray crystallographic studies confirms that the same form is present in the solid state. The stabilities and H-bond geometries of the different forms, tautomers and rotamers, are discussed by using B3LYP/6-31G** calculations. 1. Introduction.– N-Salycilidenearenamines (salicylidene = (2-hydroxyphenyl)- methylene) show hydroxyimino A/oxoenamino B tautomerism, due to a proton-trans- fer process both in solution and in the solid state (Scheme 1) [1][2]. It has been established that in aromatic Schiff bases of o-hydroxybenzaldehydes, the equilibrium, normally favoring A, is shifted toward tautomer B when increasing the polarity of the solvent or the electron-withdrawing ability of the substituent R [1]. However, the NH form B is rarely found [2] [3]. Recently, we have been involved in the study of aromatic Schiff bases derived from 3-hydroxypyridine-4-carboxaldehyde (5) in an attempt to model the H-bonded structure of the cofactor pyridoxal-5-phosphate (= 3-hydroxy-2-methyl-5-[(phos- phonooxy)ACHTUNGTRENNUNGmethyl]pyridine-4-carboxaldehyde) intervening in various enzymatic trans- formations of amino acids [4]. As a continuation of that project, we decided to exploit Scheme 1 © 2006 Verlag Helvetica Chimica Acta AG, Zürich Helvetica Chimica Acta – Vol. 89 (2006) 1290