Indian Journal Of Natural Sciences www.tnsroindia.org. © IJONS Vol.6 / Issue 35 / April 2016 International Bimonthly ISSN: 0976 – 0997 10911 Glandular Hairs and Essential Oils of Stem and Leave from the Promosing Medicinal Legume Cytisus triflorus L’ Hérit. Using Ultrasounds: Comparison with Conventional Hydrodistillation K. Ait-kaciAourahoun 1* , F. Fazouane 1 , S. Benayache 2 and F. Souici 3 1 Laboratory ofFood Technology, Faculty of Engineering Sciences, University M’HamedBouguera, 35000 Boumerdes, Algeria. 2 Research Unity (VARENBIOMOL), Department of Chemistry Faculty of Exact Sciences, Constantine 1 University, 25000 Constantine, Algeria. 3 Research and Development Center, EPE ALDAR, Moubydal Group, 16000 Algiers, Algeria. Received: 27 Jan 2016 Revised: 23 Feb 2016 Accepted: 21 Mar 2016 *Address for correspondence Aourahoun Ait-KaciKarima Department of Biology,Faculty of Sciences, M’HamedBouguera University, Boumerdes,Independence Avenue, 35000 Boumerdes, Algeria. Email: karimabio1@yahoo.fr This is an Open Access Journal /article distributed under the terms of the Creative Commons Attribution License (CC BY-NC-ND 3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. All rights reserved. Micromorphology of glandular andnon-glandular trichomes on the vegetative organs of the medicinal legume CytisustriflorusL’Hérit.were investigated using light microscopy. Essential oil composition of dry stems and leaves isolated by hydrodistillation(HD) with and without ultrasonic pre-treatment prior hydrodistillation, were analysed by GC-MS. Anatomical study revealed the presence of two-celled non- glandular trichomes and monocellularcapitate glandular hairs, which were most abundant in leave.The ultrasonic pre-treatment positively affected the leave oil yield extraction and was achieved in a reducer extraction time, whereas the relative content of target volatiles increased in stem oil with application of ultrasound prior hydrodistillation (US-HD). A total of 43 and 50 compounds were identified in the leave and stem oils of C. triflorus, respectively, in the different hydrodistillation procedures.Acids (52,48%) dominated the stem HD oil while alcohols (34,12%) majored in US-HD oil. Contrary to stem oil, both HD and HD-US leave oilswere majored by alcohols (72,93%-61,32%, respectively). The relative content increase of volatiles differed with extraction method and depended on their functional class and target compounds. This is the first report on anatomical characterization of glandular hairs from species of the genus Cytisus, and identification of oils from vegetative parts of the genus Cytisus. Key words: Essential oil, Cytisustriflorus, glandular hairs, Hydrodistillation, Ultrasounds, GC-MS, linalool, BHT, fatty acids RESEARCH ARTICLE ABSTRACT