Micromechanical Cantilever Technique: A Tool for Investigating the Swelling of Polymer Brushes Gina-Gabriela Bumbu, ²,§ Markus Wolkenhauer, ² Gunnar Kircher, ² Jochen S. Gutmann,* ,²,‡ and Ru ¨diger Berger* Max Planck Institute for Polymer Research, Ackermannweg 10, D 55128 Mainz, Germany, and Institute for Physical Chemistry, Johannes Gutenberg UniVersity, Welderweg 11, D 55099 Mainz, Germany ReceiVed July 21, 2006. In Final Form: NoVember 14, 2006 Polymer brush coatings are well-known for their ability to tailor surface properties in a wide range of applications from colloid stabilization to medicine. In most cases, the brushes are used in solution. Consequently, efforts were expended to experimentally investigate or theoretically predict the swelling behavior of the brushes in solvents of different qualities. Here, we show that the micromechanical cantilever (MC) sensor technique is a tool to perform time-resolved physicochemical investigations of thin layers such as polymer brushes. Complementary to scattering techniques, which measure the thickness, the MC sensor technique provides information about changes in the internal pressure of the brushes during a swelling and deswelling process. We show that the kinetics of both swelling and deswelling are dependent on solvent quality. Comparing the measured data with its thickness evolution, which was calculated based on the Flory-Huggins theory, we found that only the first 10% of the thickness increase of the polymer brush results in a significant pressure increase inside the polymer brush layer. Introduction Polymer molecules end-tethered to a surface with a high density of attachment points force the chains to stretch away from the solid interface, forming thin films known as polymer brushes. 1 Polymer brush layers play an important role to tailor physical and chemical properties of surfaces for applications such as colloidal stabilization, 2,3 corrosion inhibition, nonfouling surface technology, 4,5 opto-electronic devices, 6 tribology, 7 chromatog- raphy, 8 rheology, 9 chemical gates, 10 and biomedical science. 11-13 Stimuli-responsive binary polymer brush layers can be used to create switchable surfaces. 14 The properties of polymer brushes depend on their environment, e.g., the quality of a solvent. 15 Therefore, both experimental and theoretical swelling and deswelling studies of polymer brushes are mandatory to understand and tailor their properties. One way to gradually vary the solvent quality is by mixing a solvent and a nonsolvent at different ratios. Birshtein and Lyatskaya modeled the behavior of a polymer brush immersed in a mixture of miscible solvent and nonsolvent. In these studies, the Flory-Huggins interaction parameter between solvent and polymer, sp , was varied between 0 and 2. 16 Results indicated the existence of two regimes during the swelling process of the brushes in mixed solvents: the so-called “decollapse regime”, and the regime of the “swollen brush”. The “decollapse regime” shows up at low concentrations of solvent and is characterized by a small increase in the height of the brush upon increasing the solvent concentration. In this regime, the swelling is realized mainly by the penetration of the solvent molecules in the brush. The “swollen brush” regime appears at higher concentrations of solvent. Here, the increase of the brush thickness is attributed to a replacement of nonsolvent with solvent molecules. Experimentally, the swelling of polymer brushes was mainly studied by X-ray and neutron scattering techniques 17-19 pioneered by Auroy and Auvray. 17 They investigated the collapse- stretching transition of grafted poly(dimethylsiloxane) in various mixtures of dichloromethane/methanol. Knoll et al. 18 looked into the swelling behavior of polystyrene brushes with different grafting densities in mixtures of toluene/methanol of various compositions. However, the neutron scattering technique requires perdeuterated solvent mixtures to increase the contrast. This technique is not applicable for mixtures with a high proportion of solvent, since changes in Kiessig fringes could not be resolved. Therefore, the swelling of the brushes cannot be followed up to 100% solvent. 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