Cardiac Recovery in a Human Non–Heart-beating Donor After
Extracorporeal Perfusion: Source for Human Heart Donation?
Ayyaz Ali, MRCS,
a
Paul White, PhD,
b
Kumud Dhital, FRCS,
a
Marian Ryan, RGN,
a
Steven Tsui, FRCS,
a
and
Stephen Large, FRCP, FRCS
a
Successful renal, liver and more recently lung transplantation using organs from non– heart-beating donors
(NHBDs) has been reported. Regarding the heart, it has generally been assumed that warm ischemic insult would
result in overwhelming and irreversible myocardial damage. We report recovery of cardiac function in a human
NHBD by using extracorporeal perfusion 23 minutes after cardiorespiratory arrest. Successful cardiac resuscitation
in the NHBD represents a potential source of increased donor organ supply for clinical heart transplantation.
J Heart Lung Transplant 2009;28:290 –3. Copyright © 2009 by the International Society for Heart and Lung
Transplantation.
Cardiac transplantation is the definitive treatment for
end-stage heart failure. Unfortunately, a progressive
decline in the number of suitable donor organs has
limited activity worldwide.
1
Over the past decade,
there has been a progressive increase in the number
of organs procured from non– heart-beating donors
(NHBDs).
2–5
In the UK, the number of transplants using
organs from NHBDs increased by 44% in 2007.
6
It is
estimated that there are approximately 1,200 NHBDs
annually in the UK. NHBDs undergo a cardiac arrest
either in controlled or uncontrolled circumstances as
categorized by the Maastrict classification.
7
The dura-
tion of warm ischemia associated with cardiac arrest in
an uncontrolled environment (Maastricht Categories I
and II) limits the use of these organs. In the setting of
controlled cardiac arrest, after withdrawal of supportive
therapy (Maastricht Categories III and IV), access to the
donor is allowed after a mandatory “stand-off” period
during which death is confirmed. Renal, hepatic and more
recently lung transplantation has been performed success-
fully using organs from NHBDs.
8 –13
With regard to cardiac
donation it has generally been considered that warm
ischemia, with a possible contribution of anoxic neuro-
logic injury, would lead to irreversible myocardial damage.
We report successful resuscitation of a human NHBD
heart with functional recovery after rapid establishment of
extracorporeal circulation in the donor.
CASE REPORT
A 57-year-old woman presented to the local hospital with
sudden onset of headache. Her past medical history was
limited to treated hypothyroidism. Her condition deterio-
rated and she developed right-sided hemiparesis and
homononymous heminopia. A computerized tomography
(CT) scan of her brain demonstrated a large intracranial
bleed in the left parietal region, after which she was
transferred to the regional neurosurgical unit. On arrival,
she was unconscious with a Glasgow Coma Scale (GCS)
score of 3. The patient was subsequently intubated and
mechanically ventilated. She was hemodynamically stable
without inotropic support and had normal renal and
respiratory parameters. Her neurologic status was irrecov-
erable and, after discussion between her intensive-care
physicians and immediate family, it was decided to with-
draw therapy. She was a suitable candidate for non– heart-
beating organ donation and informed consent was ob-
tained by the local transplant coordinator for organ
donation and research relating to cardiac resuscitation.
The study was previously approved by our local research
ethics committee.
Mechanical ventilation was discontinued. The donor
was extubated at 3:30 p.m. and became asystolic 1
minute later. A 5-minute stand-off period was observed
to ensure the absence of any electrical cardiac activity,
allowing confirmation of death. After this period
elapsed, the donor was promptly transferred to the
operating theater where the surgical teams were
scrubbed and ready. The donor arrived into the oper-
ating theater at 3:47 p.m. and was transferred to the
operating table. A median sternotomy incision was
made at 3:50 p.m., the pericardium was opened, and
the heart and great vessels exposed. On inspection, the
heart was asystolic and moderately distended. A long
From the
a
Department of Cardiothoracic Surgery, Papworth Hospital,
Papworth Everard, Cambridge; and
b
Department of Medical Physics
and Clinical Engineering, Addenbrooke’s Hospital, Cambridge, UK.
Submitted May 20, 2008; revised September 14, 2008; accepted
December 1, 2008.
Reprint requests: Ayyaz Ali, MD, Department of Cardiothoracic
Surgery, Papworth Hospital, Papworth Everard, 10 Sandringham
Drive, Cambridge CB3 8RE, UK. Telephone: 650-669-5776. Fax:
011-44-83-1540. E-mail: ayyaz75@gmail.com
Copyright © 2009 by the International Society for Heart and Lung
Transplantation. 1053-2498/09/$–see front matter. doi:10.1016/
j.healun.2008.12.014
290
CASE REPORTS