Basic and Applied Ecology 7 (2006) 360—369 Seedling establishment after endozoochory in disturbed and undisturbed grasslands E. Cosyns a , B. Bossuyt a,Ã , M. Hoffmann a,b , H. Vervaet a , L. Lens a a Department of Biology, Ghent University, Terrestrial Ecology Unit, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium b Institute of Nature Conservation, Section Landscape Ecology and Nature Management, Kliniekstraat 25, B-1070 Brussels, Belgium Received 20 December 2004; accepted 22 August 2005 KEYWORDS Cattle dung; Gap creation; Horse dung; Safe site; Sod-cutting; Soil disturbance; Species richness Summary Local plant community composition and structure may be largely influenced by germination and seedling establishment from seeds dispersed in animal dung, through seed input, gap creation and nutrient enrichment. With an experimental approach we assessed (1) what the effect is of dung deposition on the number of seedlings in the plant community 3 months and 1 year after dung deposition, (2) what the effect is of this seedling establishment on the local plant community characteristics such as species richness and (3) if this effect interacts with large- scale soil disturbance which removes the close canopy, such as sod-cutting. Viable seeds of monocotylous species were abundantly present in the dung, and dung deposition led to a higher number of monocotylous seedlings after 3 months. However, this effect was no longer significant after 1 year. Moreover, the proportion of viable monocotylous seeds that effectively established in the field after 3 months was less than 5%. A lower number of viable seeds of the less-dominant dicotylous species was dispersed in the dung but they had a higher cover and species richness after 1 year. This resulted in an increased total small-scale species richness and diversity after dung deposition through a decreasing dominance of monocotylous species. Sod-cutting had a pronounced effect on seedling emergence: viable seeds dispersed by dung had a higher probability of successful establishment when the dung was deposited in large gaps. This indicates that an increase of safe sites associated with disturbance strengthens the effects of seed dispersal and gap creation by dung deposition. & 2006 Gesellschaft fu ¨r O ¨ kologie. Published by Elsevier GmbH. All rights reserved. ARTICLE IN PRESS www.elsevier.de/baae 1439-1791/$ - see front matter & 2006 Gesellschaft fu ¨r O ¨ kologie. Published by Elsevier GmbH. All rights reserved. doi:10.1016/j.baae.2005.08.007 Ã Corresponding author. Tel.: +3292645094; fax: +3292648794. E-mail address: Beatrijs.Bossuyt@Ugent.be (B. Bossuyt).