S214 SURVEY OF OPHTHALMOLOGY VOLUME 43 SUPPLEMENT 1 JUNE 1999 © 1999 by Elsevier Science Inc. 0039-6257/99/$19.00 All rights reserved. PII S0039-6257(99)00042-9 Systemic and Ocular Vascular Roles of the Antiglaucoma Agents -Adrenergic Antagonists and Ca 2+ Entry Blockers DAO-YI YU, PHD, MD, 1 ER-NING SU, PHD, MD, 1 STEPHEN J. CRINGLE, PHD, 1 VALERIE A. ALDER, PHD, 1 PAULA K. YU, BSc, 1 AND LOUIS DeSANTIS, PHD 2 1 Lions Eye Institute and Centre for Ophthalmology and Visual Science, University of Western Australia, Nedlands, WA, Australia, and 2 Alcon Laboratories Inc., Fort Worth, TX, USA Abstract. This review addresses whether the antiglaucoma agents -adrenergic antagonists and Ca 2+ entry blockers cause vasoactive effects in the retinal and other ocular vasculatures, as they do in other tissues. The potent vasodilating effects of Ca 2+ entry blockers on ocular vessels have recently been dem- onstrated in in vivo and in vitro studies, implying that the maintenance of ocular vascular tone relies almost exclusively on extracellular Ca 2+ . Ca 2+ entry blockers may potentially play a role in relaxing the retinal, long posterior ciliary, and ophthalmociliary arteries to improve the ocular circulation in vascu- lar diseases in which there is considerable vascular tone present. The -adrenergic antagonists are dis- cussed with reference to their antihypertensive role, their effect on other vascular beds, and finally what is known of their effect in the ocular vasculature. The emerging evidence that particular selective -adren- ergic antagonists, such as betaxolol, are also potent Ca 2+ channel entry blockers in other vascular beds is presented. Betaxolol has been shown to induce vasodilatation in the retinal and other ocular vascular beds, although studies have shown that 1 -adrenergic receptors are sparse in these vascular beds. This implies that an alternative mechanism must be responsible for betaxolol-induced vasodilatation. Evi- dence is presented that betaxolol vasodilates via its potent Ca 2+ channel entry blocking properties, and its potency and ability to vasodilate are compared with those of nimodipine and timolol, as well as with those of other Ca 2+ channel entry blockers. Important areas for future research in this area are dis- cussed. (Surv Ophthalmol 43 [Suppl 1]:S214–S222, 1999. © 1999 by Elsevier Science Inc. All rights reserved.) Key words. -adrenergic antagonists -adrenergic receptors calcium channels calcium entry blockers glaucoma long posterior ciliary artery retinal artery Ideally, potential antiglaucoma drugs should not only lower intraocular pressure (IOP), the major risk factor for glaucoma, but also improve or have no direct effect on ocular perfusion. 9,81 Certainly, drugs that reduce both IOP and ocular blood flow should not be contenders for antiglaucoma treat- ment. In recent years, Ca 2+ entry blockers have been investigated as potential antiglaucoma agents 23 and were compared with -adrenergic antagonists in current use, such as betaxolol and timolol, for their effects on the ocular circulation. In this review, the current understanding of the relative effects of Ca 2+ entry blockers and -adrenergic antagonists on ocu- lar vessels and their mechanisms of action will be dis- cussed. Vascular smooth muscle contraction and di- latation are directly regulated by intracellular Ca 2+ concentration ([Ca 2+ ] i ), which is determined by Ca 2+ entry into the cell, Ca 2+ release from intracellu- lar sites, and Ca 2+ removal. Two membrane systems play key and integrated roles in the regulation of smooth muscle [Ca 2+ ] i . These are the cell mem- brane (plasmalemma), which binds the cell and con- trols Ca 2+ movements through agonist receptor in- teraction and membrane depolarization, and the intracellular organelle, the sarcoplasmic reticulum membrane where Ca 2+ release, uptake, and move- ments are controlled by second messengers. This ar- ticle will focus on Ca 2+ movements through the plas- malemma. The Ca 2+ channels in endothelial cells, which are quite different from those in smooth mus- cle, 16 will not be covered, although the action of some vasoactive agents that modulate Ca 2+ move-