64(2014) 1 MITTEILUNGEN KLOSTERNEUBURG www.mitt-klosterneuburg.com 100 Jan 2014 RESEARCH ARTICLE ANTIPROLIFERATIVE EFFECTS OF MICROTUBULE TARGETED AGENT VINORELBINE ON CELL KINETIC OF HUMAN CERVIX CARCINOMA MEHMET TOPCUL 1 *, IDIL CETIN 2, M. Ozlem KOLUSAYIN OZAR 3 1 *Istanbul University, Faculty of Science, Department of Biology, Istanbul/Turkey. 2 Istanbul University, Institute of Science, Department of Radiobiology, Istanbul/Turkey. 3 İstanbul University, Cerrahpaşa Medical Faculty, Department of Forensic Science, Istanbul/Turkey. Corresponding Author: Mehmet TOPCUL 1 * Corresponding Author address: Istanbul University, Faculty of Science, Department of Biology, Istanbul/Turkey. Corresponding Author e-mail address: topcul@istanbul.edu.tr Summary In this study, the antiproliferative effects of vinorelbine which belongs vinca alkaloids family and is used as an anti-microtubule agents was evaluated on HeLa cell line originated from human cervix carcinoma. For this purpose cell kinetic parameters including cell viability analysis, mitotic index, labelling index and apoptotic index were used. The results showed that while there was a significant decrease in rates of viability and labelling index values, there was a significant increase in mitotic index values regarding the cell cycle effects depending on G 2 /M block for all experimental groups. Also there was a significant increase in apoptotic index values depending on interfering with the dynamics of microtubules. Differences between control and all experimental groups were statistically significant (p<0.01) for all applications. Key words: Cervix cancer, Vinorelbine, Cell Viability, Mitotic Index, Labelling Index, Apoptotic Index Introduction Microtubules are a major component of the cytoskeleton. They are important players in many cellular events and play a crucial role in cell division. As such, microtubules are a highly attractive target for anticancer drug design. The tubulin-binding agents