Results and discussion: These malformations are the most common types of birth defects and represent an extremely complex biomedical problem. They are specific morphogenetic processes that are easily detectable, but appear to be a complex multifactorial trait. These malformations might be due to an uncontrolled migration of neural crest cells, which causes other developmental disorders. Discussion: These data indicated that a correlation between SM exposure and teratogenic activities does exist. The potential modifying roles of various genes with the environment are considered too. Keywords: Sulfur Mustard, Teratogenicity, Malformation doi:10.1016/j.clinbiochem.2011.08.990 Modern Techniques and Biomedical Diagnosis Poster – [A-10-89-2] Fabrication of a novel nanocomposite based on sol–gel process for hollow fiber-solid phase microextraction of Atnelol in biological samples combined with HPLC-DAD determination Fateme Samadi a , Zarrin Eshaghi b , Mahmood Ebrahimi a , Farida Zohuorian a , Armin Oskoueian c , Mahdi Moghadam Poor d , Ali Samadi d a Faculty of Science, Islamic Azad University, Mashhad Branch, Iran b Faculty of Science, Payame Noor University, Mashhad Branch, Iran c Faculty of Agriculture, Islamic Azad University, Mashhad Branch, Iran d Faculty of Power, Islamic Azad University, Gonabad Branch, Iran E-mail addresses: fsamadi6258@gmail.com (F. Samadi), armin_oskoueian@yahoo.com (A. Oskoueian) Introduction: This research provided an overview of the new developments in material and format technology that improve the extraction of semi-polar compounds in several extraction techniques. It mainly included a novel solid-phase microextraction, that uses a simple carbon nanotube reinforced sol–gel in-fiber device. The simple device was used for extraction, pre-concentration and determination of Atnelol in biological samples. In this new technique carbon nanotube reinforced sol was prepared by the sol–gel method via the reaction of tetraethoxysilane with a basic catalyst. Material and methods: The sols were prepared by the basic catalyzed method from TEOS and Tris hydroxymethyl aminoethan in EtOH. Then mixture was stirred for one day .Then NH4OH , multiwalled carbon nanotubes and HCL were added to mixture and stirred for 10 min. Results: The effective extraction parameters were optimized carefully as follows. The proposed extraction procedure was carried out at different times. From these results, it was concluded that equilibrium is reached during 20 min. Experimental studies showed that the best extraction efficiency is obtained for 25 mL of analyte as the donor volume. Conclusion: A new, simple, rapid and compatible method has a high enrichment factor and excellent selective clean up of sample. Good linearity and reasonable relative recovery were also obtained. It is the method for the fast, trace analysis in many complicated matrixes such as environmental and biological samples. We used the method to isolate Atnelol from real samples and found it to have many advantages over conventional methods. Keywords: Atnelol, Carbon nanotube, Sol–gel, HPLC, Solid phase microinstruction doi:10.1016/j.clinbiochem.2011.08.991 Poster – [A-10-146-1] Effect of medium composition fermentation conditions on erythromycin production by Saccharopolyspora erythraea Samaneh Yavari, Razieh Rafieenia School of Biological Sciences, University of Reading, Reading, UK E-mail addresses: dr81_yavari@yahoo.com (S. Yavari), r.rafieenia@gmail.com (R. Rafieenia) Erythromycin is a polyketide antibiotic produced by Saccharopoly- spora erythraea mainly in submerged culture. In this paper the effect of important culture components such as carbon, nitrogen and phos- phorous sources, and also culture conditions like temperature, medium pH, aeration and morphology on erythromycin production have been reviewed. Saccharopolyspora erythraea is a member of the actinomycetes group and grows through the formation of long multicellular hyphae with a hyphal diameter of 0.4–0.8 μm. It's optimum growth is in 28–38 °C. Nowadays, the industrial production of erythromycin is mainly carried out by Saccharopolyspora erythraea at the end of the trophophase. In general, for effect of medium composition on Erythromycin production, carbon source is used in relatively higher concentration compared to other medium component and the formation of many antibiotics and other secondary metabo- lites is suppressed by rapidly used carbon sources. For nitrogen source, ammonium salts exert a negative effect on the production of many antibiotics including erythromycin. Excess ammonium in culture suppress the production of many antibiotics. Among ammonium salts, ammonium sulfate is the best source. According to the results, it is not preferable to substitute the inorganic nitrogen source in the medium with organic source and the best form of nitrogen source is using both inorganic (ammonium sulfate) and organic nitrogen source (CSL) in medium. Also, phosphate is the crucial growth limiting nutrient in many antibiotic fermentations. Phosphorus is a key element in many important molecules including nucleic acids and polyphospholipids phosphate in concentrations ranging from 0/3 to 300 Mm generally support extensive cell growth, but concentrations of 10 Mm and above suppress the biosynthesis of many antibiotics. Also culture conditions can be based on: temperature (33 °C), pH of medium (6/8), aeration and morphology (diameter>80–90 μ). For conclusion, this study introduces the key factors in cultures of Saccharopolyspora erythraea for erythromycin production. Studies have shown that using cheap carbon (cane molasses and starch) and nitrogen (CSL and soybean mill) sources decrease raw material costs and also increase the production of erythromycin; so their use in industrial processes is advantageous economically. The pH and temperature of culture should be regulated in optimum values to reach high productivity. Productiv- ity of the cultures also relates to mean diameter of the minimum bounding circle of the hyphal fragments in the culture. A minimum bounding circle diameter was necessary for a hyphal fragment to be productive that is 88 μ for erythromycin production by Saccharopoly- spora erythraea. In summary, recognition of important factors affecting on Erythromycin production can help to design industrial processes for economic production of this important drug. Keywords: Erythromycin production, Saccharopolyspora erythraea, Culture medium, Fermentation conditions doi:10.1016/j.clinbiochem.2011.08.992 Poster – [A-10-201-2] Comparison of comon methods of luminol immobilization on nanoparticles Soheila Sabouri, Mostafa Shourian, Hedayatollah Ghourchian Institute of Biochemistry and Biophysics, Faculty of Science, Tehran University, Tehran, Iran Abstracts S310