P2.95. Hyperthermia using magnetic nanoparticles combined with intratumoral dendritic cells enhance antitumor effect K. Matsumoto *, N. Yamamoto, H. Furue, D. Mizuno, F. Sato, T. Shigetomi Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Japan Dendritic cells (DCs) are potent antigen-presenting cells that play a pivotal role in regulating immune responses in cancer and have recently been shown to be activated by heat shock proteins (HSPs). We previously reported that HSP70 expression after hyperthermia induces antitumor immunity. Our hyperthermia system using mag- netite cationic liposomes (MCLs) induced necrotic cell death that was correlated with HSP70 release The aim of this study was to investigate the antitumor effect of combined therapy ‘‘hyperthermia and DCs” for oral spuamous cell carcinoma(SCC). In this study, we used C3H/HeJ mice and mouse SCC cell line (SCCVII). Immature DCs were cultured for 10 days with GM-CSF and IL-4. Immature DCs and were pulsed with LPS or mouse SCCVII cells heated at 43 °C, were analyzed by FACScan flow cytometry. Major histocompatibility complex (MHC) class II, costimulatory molecules CD86 in the DCs pulsed with LPS and heated SCCVII cells were upreg- ulated, thus resulting in DC maturation. In an vitro study, exponen- tially growing SCCVII cells(5 Â 10 6 ) were immersed into a temperature-controlled water bath with various temperature, time or frequency to inbestigate optimal cell killing and HSP70 release. Release of HSP70 was assayed by Western blotting. C3H/HeJ mice bearing a SCCVII nodule were subjected to combi- nation therapy using hyperthermia and DC immunotherapy in vivo by means of tumor-specific hyperthermia using MCLs and directly injected immature DCs. Mice were divided into 4 groups: group I (control), group II (hyperthermia), group III (DC therapy) and group IV (hyperthermia + DC therapy). Inhibition and complete regression of tumors were observed in group IV mice. Groups II and III had slight inhibition of tumors. Increased cytotoxic T lymphocyte (CTL) activity was observed on in vitro cytotoxicity assay using spleno- cytes in the cured mice treated with combination therapy. This study has important implications for the application of MCL-induced hyperthermia plus DC therapy in patients with advanced oral SCC as a novel cancer therapy. doi:10.1016/j.oos.2009.06.499 P2.96. Effectiveness of the use of hyperthermy in the combined treatment of cancer of tong and bottom of cavity of mouth I.V. Reshetov*, O.V. Matorin, A.V. Koritskiy Moscow P.A. Hertzen Cancer Research Institute, Russian Federation We have available the experience of the treatment of 30 patients of them by 15 with the localization of the tumor process in the region of language and 15 in the region of the bottom of the cavity of mouth. The primary histology of tumor was evaluated taking into account data of ultrasonic inspection, CT and biopsy. On histogenesis of tumor process all – planocellular cancer, from them 21 man and 9 women. On the age classes the patients were distributed as follows from 34 to 78 years. The patients were distributed as follows in the stages: 2 stages – 18, 3 stages – 12. According to the nature of tumor process primary tumors comprised – 22, relapses – 8. Type of the previous treatment composed: 12 – the radiation therapy (preoper- ation – 11, according to the radical program – 4). Depending on the prevalence of the tumor process of interference they were carried out in 10 cases under the overall endotracheal anesthesia, into 20 under the local infiltration anesthesia with the involution. The per- iod of observation in this group is from 3 to 24 months. Stabilization Regression Continued Increase Primary tumor 16 2 Relapse 8 4 The obtained first positive clinical results of the combined treat- ment of patients with the malignant tumors of tong and bottom of the cavity of mouth with the use of a radio-wave complex ‘‘Met- atom-2” testify about the prospect of method. The wide spectrum of the possibilities of instrument, the variety of electrodes make it possible to use it with different sizes and localizations of tumor pro- cess both in the independent version and in the combination with the standard procedures of beam and chemotherapy. As a whole an improvement in the results of treating the sick localizations indi- cated must be examined in the plane of integral approach and the procedure of radio-frequency thermo-ablation can become one of the important contemporary it is sectional in the therapeutic chain. doi:10.1016/j.oos.2009.06.500 P2.97. Definite, probable or dubious: Antioxidants trilogy in oral disease management G. Rodrigues* Kasturba Medical College, Manipal, India Recent years have shown the presence of free radicals in causa- tion and progression of various oral diseases and antioxidant usage in oral therapeutics. Antioxidant micronutrients are important not only for limiting oxidative and tissue damage, but also in preventing increased cytokine production, which is a result of prolonged activa- tion of the immune response. There has been a widespread availabil- ity and clinical claims on the safety and efficacy of antioxidants. However, lack of randomized controlled trials in clinical research limits their usage. There is a substantial gap between these clinical claims and efficacy/safety of antioxidants in oral therapeutics which needs to be addressed. This poster highlights the clinical significance of antioxidant ther- apy in clinical practice and touches upon controversial areas and research lacunae. An evidence based approach for making recom- mendations about antioxidant usage and efficacy in routine clinical practice seems to be the need of the hour. doi:10.1016/j.oos.2009.06.501 P2.98. Tetrathiomolybdate inhibits head and neck cancer metastasis by decreasing tumor cell invasion and promoting tumor cell anoikis P. Kumar a,b, *, S.N. Patel c , M. Islam a,b , Q. Pan a,b , S. Merajver c , T.N. Teknos a,b a The Ohio State University, United States b James Comprehensive Cancer Center, United States c University of Michigan, United States Objective: The metastatic spread of solid tumors is directly or indirectly responsible for most cancer-related deaths. Growing evi- dence suggests that tumor progression is dependant on ‘‘angiogenic 192 Poster session II / Oral Oncology Supplement 3 (2009) 162–200 Committee Listings Welcome IAOO Program Keynote Bios. Keynote Abs. Pan. Disc. & Symp. Abs. Orals List Poster List Oral Abstracts Poster Abstracts