Abstracts / Toxicology Letters 205S (2011) S60–S179 S133 P1239 Influence of abiotic factors in the cultivation of Mysidopsis juniae T.M.N.D. Oliveira 1,∗ , C. Vaz 1 , T. Kleine 2 , W.G. Matias 3 , R.F.S. Böhm 3 , R.A. Gonc ¸ alves 4 , T.S. Tortelli 4 , V.G. Barros 1 1 Environmental Engineering, University of Joinville Region - Univille, Joinville, Brazil, 2 Health and Environment Master Programm, University of Joinville Region - Univille, Joinville, Brazil, 3 Sanitary and Environmental Engineering, Federal University of Santa Catarina, Florianópolis, Brazil, 4 Marine Biology, University of Joinville Region - Univille, Joinville, Brazil Purpose: This study was developed to determine the salinity and temperature conditions needed for the maintenance and cultiva- tion of Mysidopsis juniae in the laboratory. Methods: To conduct this study, we created two factorial designs. The salinities tested in the first and second experiment were 25, 28, 32 and 35 with temperatures of 10, 17, 22, 25, 30 and 32 ◦ C, respectively. All parameters were monitored daily with a maxi- mum variation of ±1 and ±1 ◦ C. The samples were prepared from deionized water with the addition of Red Sea salt. For each sample, we used 60 mysids that were 5–7 days old and that were cultivated in the laboratory. Temperatures of 28, 30 and 32 ◦ C were main- tained with the use of an incubator, temperatures of 10 and 17 ◦ C were maintained with a refrigerator and temperatures of 22 and 25 ◦ C were maintained by air conditioners. The photoperiod was 12 h dark/12 h light. The mysids were fed with Artemia sp. that were enriched with fish oil and liver oil from codfish. Results: The survival and reproduction of the organisms was influenced by salinity and temperature, with a salinity of 32 and a temperature of 25 ◦ C being the most suitable conditions. At tem- peratures of 10, 17 and 30 ◦ C, all organisms died after 21 days of testing. There were similar results for salinities of 25, 28 and 35. We suggest the use of a salinity of 33 ± 1 and a temperature of 24 ± 1 ◦ C for the cultivation of Mysidopsis juniae in the laboratory. doi:10.1016/j.toxlet.2011.05.473 P1240 Exposure to mites, sensitisation and allergy to mites in moisture damaged buildings S. Pennanen Work Environmental Development, Finnish Institute of Occupational Health, Kuopio, Finland The aim of our study was to estimate mite exposure in mouldy buildings both quantitatively and the species level and to assess respiratory and general symptoms in people with work- or home- related exposure in moisture-damaged buildings. We assessed the mite exposure of patients in the Skin and Allergy Hospital by col- lecting samples of settled dust from their workplaces and homes. Mites were counted and identified microscopically. Mites were found in 26% of the samples. Three samples (3%) contained more than 100 mites per gram dust and another sample (1%) had more than 500 mites per gram dust, the suggested limits for mite sen- sitisation and at which acute allergic symptoms can be expected, respectively. Mites were a more common finding from water dam- aged houses or homes and workplaces with visible mould but they also existed in large numbers in buildings with no reported indoor air quality problems. Dust mites (Pyroglyphidae) were the most abundant species. However, storage mites were also frequently found. Patients reported a high prevalence of respiratory symp- toms, especially nose and eye irritation which may partly be due to sensitisation especially to storage mites. Although mites are com- monly found in workplaces and homes they are seldom present in high concentrations. Mite sensitisation and especially a positive reaction to storage mites in skin prick tests may be an indication of a moisture problem in the domestic or work environments. doi:10.1016/j.toxlet.2011.05.474 P1241 Effects of subchronic exposure to triazines on Danio rerio L. Plhalova ∗ , I. Haluzova, S. Macova, P. Dolezelova, P. Marsalek, Z. Svobodova, V. Pistekova, I. Bedanova University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic The aim of this study was to investigate effects of subchronic exposure to sublethal levels of some triazines (atrazine, simazine and terbutryn) on growth and on histopathological changes in Danio rerio. Triazines form a group of similar herbicides used exten- sively in agriculture and non-agricultural use sites, primarily to control broadleaf and some grassy weeds, that have become ubiq- uitous contaminants of the environment. The juvenile growth tests were performed on D. rerio according to the OECD method No. 215. Fish at the age of 20 days were exposed for 28 days to the atrazine, simazine and terbutryn environmental concentrations commonly detected in the Czech rivers and the range of sublethal concen- trations of these triazines. The environmental concentrations of atrazine, simazine and terbutryn in the Czech rivers did not have any effects on growth and on histopathological changes in D. rerio and this concentration is lower than determined values of LOEC (90 gl -1 of atrazine, 60 gl -1 of simazine and 0.6 mg l -1 of ter- butryn) and NOEC (30 gl -1 of atrazine, 6 gl -1 of simazine and 0.2 mg l -1 of terbutryn) of these triazines. This research was supported by the Ministry of Education, Youth and Sports of the Czech Republic (MSM Project No. 6215712402) and IGA 199/2009/FVHE, IGA VFU No. 87/2010/FVHE. doi:10.1016/j.toxlet.2011.05.475 P1242 Effect of travel restriction on PM 10 concentrations in Naples G. Polichetti 1,∗ , D. Capone 1 , K. Grigoropoulos 2 , R. Polichetti 3 , C. Attianese 1 , A. Nunziata 4 , A. Gentile 1 1 Department of Neurosciences, Unit of Clinical Pharmacology, University of Naples, Naples, Italy, 2 Geology and Environment, University of Patras, Patras, Greece, 3 Department of Neurological Sciences, University of Naples, Federico II, Naples, Italy, 4 Pomezia, Italy Purpose: In 2010 the city of Naples established the travel restric- tion for the cars belonging to the classes Euro 0–3. For the cars belonging to the classes 2 and 3 it was allowed to travel if they have on board at least 3 passengers, while to all cars it is allowed to travel if provided of methane or LPG system. The restriction was valid from 7.30 to 10.30 am on Monday, Wednesday and Friday. Methods: Using the data supplied by the A.R.P.A.C., we compared the PM 10 concentrations obtained from 8.00 to 10.00 am in the days with and without restriction during the period from April to July 2010. In addition, we compared also the PM 10