ISSN 0096-3925, Moscow University Biological Sciences Bulletin, 2010, Vol. 65, No. 3, pp. 98–103. © Allerton Press, Inc., 2010.
Original Russian Text © A.A. Sinyushin, 2010, published in Vestnik Moskovskogo Universiteta. Biologiya, 2010, No. 3, pp. 11–16.
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INTRODUCTION
The investigation of developmental abnormalities,
teratology, has recently obtained a somewhat different
meaning as compared with a period of its origin.
Except function of direct description of certain terato-
logical phenomena, this part of morphology entered a
field of modeling of developmental processes, muta-
tion analysis, and even evolutionary reconstructions in
light of the Evo-Devo concept. Teratology itself has
tightly associated with contemporary molecular devel-
opmental genetics, the latter significantly pushing out
the structural approach in understanding of morpho-
logical abnormalities.
Fasciation represents one of the most widespread
anomalies of plant development. One may propose
that the main problem in discussion of this phenome-
non is absence of satisfactory definition. Numerous
authors give similar and, at the same time, different
characteristics [1, 2].
Studies of model objects, mainly Arabidopsis
thaliana (L.) Heynh. (Brassicaceae), give a certain key
towards understanding this problem. Genetic control
of formation and specialization of stem apical mer-
istem (SAM) is studied precisely enough. It had been
demonstrated that basic mechanism of regulation of
SAM activity is negative feedback between gene WUS-
CHEL (WUS) and multiple negative regulators of its
expression (CLAVATA (CLV), FASCIATA (FAS) etc.)
[3]. Mutations in these genes lead to overexpression of
WUS in SAM and floral meristem (FM); as a result,
abnormal enlargement of this meristem occurs,
namely fasciation. However, investigations on varia-
tions on fasciation in different taxa clearly demon-
strate how many different transformations the basic
model (designed for model object with simple inflo-
rescence and actinomorphic flower) can undergo.
Many plants are known to possess inherited forms of
fasciation affecting SAM only and not FM. Works in
which structure of abnormal organs could be analyzed
from the positions of molecular developmental genet-
ics are obviously lacking. The most detailed review
devoted to fasciation is still the work of O.E. White [1].
The structural approach seems very important when
combined not only with modern molecular systemat-
ics [4], but also with contemporary developmental
genetics.
The flower, an organ with discrete structure com-
posed of parts with more or less constant number and
position, represents a convenient model for studies on
mechanisms of fasciation. The given work is aimed at
generalization of material dealing with flower fascia-
tion, estimation of mechanisms underlying it and for-
mulation of more or less comprehensive definition of
the given phenomenon.
MATERIALS AND METHODS
The living or fixed in 70% ethanol material was
used for investigations on flower morphology. Flowers
and inflorescences of pea line “Lupinoid” were col-
lected in 2005–2007 from plants grown on a territory
of Zvenigorod Biological Station of Moscow State
University (Odintsovo raion, Moscow region). Flow-
ers of Allium nutans L., A. cepa L., and Lupinus poly-
phyllus Lindl. were collected in 2006–2007 in Ruza
raion of Moscow region as components of cultural
flora. Fruits of Alisma plantago-aquatica L. were col-
lected in 2005 on a territory of “Zhuravlinaya rodina”
natural preserve (Taldom raion, Moscow region).
Flowers of Syringa vulgaris L. (wild lilac and cv. Paul
Hariot) were collected in 2007 in the Botanical Gar-
den of Moscow State University. The material fixed in
ethanol was prepared for scanning electron micros-
copy (SEM) according to protocol described in work
Flower Fasciation:
I. Origin of Enlarged Meristem
A. A. Sinyushin
Genetics Department, Moscow State University, Moscow, Russia
e-mail: sinjushin@pisumsativum.org
Received May 5, 2009
Abstract—Different ways of formation of fasciated floral meristem were analyzed. It has been demonstrated
that distinguishing between phenomena of enlargement of a single growing point and fusion of a few into sin-
gle one is impossible. The frequency of abnormalities is proposed as criterion for such classification.
Key words: flower, fasciation, floral meristem
DOI: 10.3103/S009639251003003X